Mouse Mucin-5 subtype AC,MUC5AC ELISA Kit
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中文名稱：小鼠粘蛋白/粘液素5AC(MUC5AC)酶聯(lián)免疫試劑盒
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貨號：CSB-E11040m
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠粘蛋白/粘液素5AC(MUC5AC)酶聯(lián)免疫試劑盒（CSB-E11040m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MUC5AC含量。MUC5AC是一個重要靶點(diǎn)。它是一種黏蛋白，與呼吸系統(tǒng)等疾病密切相關(guān)，如哮喘、慢性阻塞性肺疾病等。研究機(jī)制主要聚焦于其在疾病發(fā)生發(fā)展中參與調(diào)節(jié)黏液分泌、影響炎癥反應(yīng)等，對它的深入研究有望為相關(guān)疾病治療提供新策略。試劑盒檢測范圍為4.688 ng/mL-300 ng/mL，本試劑盒適用于體外實(shí)驗(yàn)研究，如評估病理狀態(tài)下粘液分泌調(diào)節(jié)機(jī)制、篩選影響粘蛋白表達(dá)的化合物或藥物，以及探究感染性疾病中宿主防御反應(yīng)等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：4.688 ng/mL-300 ng/mL
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靈敏度：1.172 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-92 1:2 Average % 90 Range % 85-95 1:4 Average % 98 Range % 91-102 1:8 Average % 96 Range % 93-103 -
回收率：
The recovery of mouse MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 91-104 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 300 2.275 2.182 2.229 2.057 150 1.463 1.434 1.449 1.277 75 0.855 0.818 0.837 0.665 37.5 0.524 0.507 0.516 0.344 18.75 0.351 0.329 0.340 0.168 9.375 0.281 0.262 0.272 0.100 4.688 0.235 0.217 0.226 0.054 0 0.174 0.169 0.172 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Discovery of Fungus-Derived Nornidulin as a Novel TMEM16A Inhibitor: A Potential Therapy to Inhibit Mucus Secretion in Asthma P Pongkorpsakol,Journal of experimental pharmacology,2023
- Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway MA Jung,Biomedicine & pharmacotherapy,2023
- Alnus hirsuta (Spach) Rupr. Attenuates Airway Inflammation and Mucus Overproduction in a Murine Model of Ovalbumin-Challenged Asthma Ji-Hye Ha,Frontiers in Pharmacology,2021
- Combined treatment with SB203580 and dexamethasone suppresses non-typeable Haemophilus influenzae-induced Th17 inflammation response in murine allergic asthma Wang G, et al,European Journal of Pharmacology,2019
- Chronic intermittent hypoxia decreases pulmonary clearance of 99mTc-labelled particulate matter in mice Fu C.et al,Am J Transl Res,2017
- The immune profile associated with acute allergic asthma accelerates clearance of influenza virus Samarasinghe AE et al,Immunol Cell Biol,2014
- Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice /,PLOS ONE,2012
- A Dissociated Glucocorticoid Receptor Modulator Reduces Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma /,The Journal of Immunology,2012
產(chǎn)品評價(jià)
樣品類型：組織勻漿液 肺組織勻漿上清
樣品信息：小鼠
稀釋比：沒有稀釋
產(chǎn)品評價(jià)： 我通過使用CSB-E11040m檢測了PR8病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結(jié)果十分精準(zhǔn)
By 鄧?yán)蠋?/p>
樣品類型：組織勻漿液 肺組織勻漿上清
樣品信息：小鼠
稀釋比：1:100
產(chǎn)品評價(jià)： 我通過使用CSB-E11040m檢測了流感病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結(jié)果十分精準(zhǔn)
By 鄧?yán)蠋?/p>
樣品類型：其他樣品處理方法 肺泡灌洗液
樣品信息：小鼠
稀釋比：沒有稀釋
產(chǎn)品評價(jià)： 我用CSB-E11040m檢測正常和吸煙的C57B/L小鼠肺泡灌洗液MUC5AC的水平，正常小鼠的OD值在0.061-0.415，吸煙小鼠的OD值在0.652-0.776，結(jié)果符合組織PAS染色結(jié)果。操作簡單方便，推薦！
By 肖老師
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