Rat Laminin,LN ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠層連蛋白/板層素(LN)酶聯(lián)免疫試劑盒（CSB-E04646r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的LN含量。大鼠層連蛋白/板層素（LN）是細(xì)胞外基質(zhì)的主要結(jié)構(gòu)成分，對基膜的組裝起關(guān)鍵作用。其在細(xì)胞表面形成網(wǎng)絡(luò)結(jié)構(gòu)，將細(xì)胞固定在基膜上，參與多種生理過程，如細(xì)胞粘附、增殖和分化。研究顯示，LN在肝硬化等疾病中表達(dá)增加，可能與肝竇毛細(xì)血管化形成有關(guān)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中LN相關(guān)分子機(jī)制探索，如肝/肺纖維化模型評估、傷口愈合過程監(jiān)測、腫瘤侵襲性研究等，為體外實驗提供可靠的蛋白定量工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

LN
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-99
1:200	Average %	97
	Range %	93-105
1:400	Average %	85
	Range %	82-89
1:800	Average %	90
	Range %	85-98

回收率：

The recovery of rat LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	89-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.