Mouse Laminin,LN ELISA kit
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中文名稱:小鼠層連蛋白/板層素(LN)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E04645m
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規(guī)格:96T/48T
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價(jià)格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠層連蛋白/板層素(LN)酶聯(lián)免疫試劑盒(CSB-E04645m)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的LN含量。小鼠層連蛋白(LN)是一種細(xì)胞外基質(zhì)蛋白,參與細(xì)胞粘附、遷移和細(xì)胞信號(hào)傳導(dǎo)。其功能背景涉及多種生理過程,如胚胎發(fā)育、組織修復(fù)和腫瘤生長(zhǎng)。研究機(jī)制主要聚焦于LN與細(xì)胞表面受體相互作用,以及其在細(xì)胞骨架形成和細(xì)胞間通訊中的作用。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域,如探索組織再生機(jī)制、纖維化病理模型(如肝/肺纖維化)的分子標(biāo)志物動(dòng)態(tài)變化,或體外培養(yǎng)細(xì)胞分泌LN的功能研究,為相關(guān)疾病機(jī)制解析及藥物干預(yù)效果評(píng)估提供可靠工具。試劑盒支持多種生物樣本類型,滿足不同實(shí)驗(yàn)場(chǎng)景需求,且操作流程簡(jiǎn)便,重復(fù)性良好,適用于實(shí)驗(yàn)室常規(guī)檢測(cè)與高通量篩選。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:N/A
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縮寫:LN
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 99 Range % 94-102 1:2 Average % 87 Range % 84-90 1:4 Average % 91 Range % 88-96 1:8 Average % 101 Range % 97-105 -
回收率:
The recovery of mouse LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 98 94-102 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.407 2.469 2.438 2.281 50 1.472 1.502 1.487 1.330 25 0.909 0.935 0.922 0.765 12.5 0.597 0.578 0.588 0.431 6.25 0.364 0.378 0.371 0.214 3.12 0.274 0.298 0.286 0.129 1.56 0.209 0.211 0.210 0.053 0 0.158 0.156 0.157 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy F Wang,Journal of diabetes research,2024
- SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,2018
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