Rat Interleukin 5,IL-5 ELISA KIT

產品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠白介素5(IL-5)酶聯免疫試劑盒（CSB-E07435r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL5含量。IL-5 是一種重要的細胞因子，在嗜酸性粒細胞的生長、分化及存活中發揮關鍵作用。其過度表達與多種炎癥性疾病如哮喘、嗜酸性粒細胞增多癥等相關。研究聚焦于阻斷 IL-5 信號通路，以減少嗜酸性粒細胞數量，從而緩解相關疾病的炎癥癥狀。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研領域中IL-5相關免疫調節機制研究，如構建哮喘動物模型時評估炎癥因子水平，或體外藥物篩選實驗中監測細胞因子分泌動態。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
縮寫：

IL5
Uniprot No.：

Q08125
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-91
1:2	Average %	92
	Range %	83-99
1:4	Average %	101
	Range %	93-107
1:8	Average %	96
	Range %	90-104

回收率：

The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-94
EDTA plasma (n=4)	94	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
亞細胞定位：

Secreted.
蛋白家族：

IL-5 family
數據庫鏈接：

STRING: 10116.ENSRNOP00000010729

UniGene: Rn.44227