Rat 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit
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中文名稱：大鼠8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E10526r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠8羥基脫氧鳥(niǎo)苷(8-OHdG)酶聯(lián)免疫試劑盒（CSB-E10526r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的8-OHdG含量。8-OHdG（8-羥基脫氧鳥(niǎo)苷）是DNA中的一種氧化損傷產(chǎn)物，由活性氧自由基攻擊DNA分子中的鳥(niǎo)嘌呤堿基第8位碳原子產(chǎn)生。它是氧化應(yīng)激和DNA損傷的重要生物標(biāo)志物，與多種疾病如癌癥、心血管疾病和神經(jīng)退行性疾病有關(guān)。研究8-OHdG有助于了解DNA損傷和修復(fù)過(guò)程，以及評(píng)估疾病風(fēng)險(xiǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于體外細(xì)胞模型、動(dòng)物實(shí)驗(yàn)中的氧化應(yīng)激評(píng)估，如藥物抗氧化效果驗(yàn)證、環(huán)境污染因子（如重金屬、輻射）的DNA損傷研究，以及腦組織、肝臟等器官的氧化損傷機(jī)制分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：8-OHdG
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：0.312 ng/mL-20 ng/mL
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靈敏度：0.078 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cell Biology
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 88-98 1:2 Average % 96 Range % 91-102 1:4 Average % 90 Range % 85-96 1:8 Average % 96 Range % 88-99 -
回收率：
The recovery of rat 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 98-107 EDTA plasma (n=4) 96 90-102 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 1.890 1.780 1.835 1.682 10 1.198 1.128 1.163 1.010 5 0.756 0.726 0.741 0.588 2.5 0.525 0.495 0.510 0.357 1.25 0.321 0.318 0.320 0.167 0.625 0.272 0.262 0.267 0.114 0.312 0.213 0.209 0.211 0.058 0 0.154 0.152 0.153 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Repurposing the antimalarial chloroquine: a potential therapy for hepatic injury in a rat model of hindlimb ischemia–reperfusion by modulating apoptosis, autophagy, inflammation, and oxidative stress MM Sherif, HS El-Abhar, HM Fawzy,Future Journal of Pharmaceutical Sciences,2025
- Metabolomics analyses and comparative insight to neuroprotective potential of unripe fruits and leaves of Citrus aurantium ethanolic extracts against cadmium-induced rat brain dysfunction: involvement of oxidative stress and akt-mediated CREB/BDNF and GSK DAH Deabes, EAW El-Abd, SM Baraka,Metabolic Brain Disease,2025
- Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair Y Xiao,Nano-micro letters,2024
- Edible wild plants, chicory and purslane, alleviated diabetic testicular dysfunction, and insulin resistance via suppression 8OHdg and oxidative stress in rats EA Saad,PloS one,2024
- Mechanistic aspects of ameliorative effects of Eicosapentanoic acid ethyl ester on methotrexate-evoked testiculopathy in rats NAT Abbas,NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY,2024
- Esculetin and Fucoidan Attenuate Autophagy and Apoptosis Induced by Zinc Oxide Nanoparticles through Modulating Reactive Astrocyte and Proinflammatory Cytokines in the Rat Brain WJ Song,Toxics,2023
- Anti-radiation effect of MRN-100: a hydro-ferrate fluid, in vivo Y Xue,Journal of radiation research,2024
- Gastroprotective effect of dapagliflozin in ethanol-induced gastric lesions in rats: Crosstalk between HMGB1/RAGE/PTX3 and TLR4/MyD88/VEGF/PDGF signaling pathways RM Salama,International immunopharmacology,2023
- Neuroprotective effect of peanut against oxidative stress in streptozotocin-induced diabetic rats NH Mohamed,Egyptian Journal of Basic and Applied Sciences,2022
- Hydrogen exerts neuroprotective effects by inhibiting oxidative stress in experimental diabetic peripheral neuropathy rats XC Han,Medical Gas Research,2022
- The Effect of Metformin in Diabetic and Non-Diabetic Rats with Experimentally-Induced Chronic Kidney Disease MA Za'abi,Biomolecules,2021
- Graphene oxide induces dose?dependent lung injury in rats by regulating autophagy Mingke Qiu,Experimental and Therapeutic Medicine,2021
- β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake Z Chen,Archives of Pharmacal Research,2020
- Protective Effects of Hyperbaric Oxygen Therapy on Brain Injury by Regulating the Phosphorylation of Drp1 Through ROS/PKC Pathway in Heatstroke Rats Ni XX,Cell Mol Neurobiol,2020
- Resveratrol improves left ventricular remodeling in chronic kidney disease via Sirt1-mediated regulation of FoxO1 activity and MnSOD expression Li P, et al,Biofactors,2019
- Isoorientin plays an important role in alleviating Cadmium-induced DNA damage and G0/G1 cell cycle arrest Shihao Chen, et al,Ecotoxicology and Environmental Safety,2019
- Galangin Activates Nrf2 Signaling and Attenuates Oxidative Damage, Inflammation, and Apoptosis in a Rat Model of Cyclophosphamide-Induced Hepatotoxicity Aladaileh SH, et al,Biomolecules,2019
- Amelioration of fenitrothion induced oxidative DNA damage and inactivation of caspase-3 in the brain and spleen tissues of male rats by N-acetylcysteine Rasha T. Alam,Life Sciences,2019
- The beneficial effect of α-tocopherol succinate supplementation on DNA oxidation induced by intensive exercise training Hossein TaheriChadorneshin, et al,Sport Sciences for Health,2018
- Aplysin Protects Against Alcohol┸\Induced Liver Injury Via Alleviating Oxidative Damage and Modulating Endogenous Apoptosis┸\Related Genes Expression in Rats Na Ge.et al,Journal of Food Science,2018
- PM2.5 induces male reproductive toxicity via mitochondrial dysfunction, DNA damage and RIPK1 mediated apoptotic signaling pathway Jin Zhang.et al,Science of The Total Environment,2018
- The Ameliorative Potential of Dexmedetomidine and Benincasa Cerifera Extract in Renal Ischemia/Reperfusion Injury in a Streptozotocin-Induced Diabetic Model Gehan A Hegazy.et al,Biomedical and Pharmacology,2018
- Puerarin Mitigates Diabetic Hepatic Steatosis and Fibrosis by inhibiting TGF-B signaling pathway activation in Type 2 Diabetic Rats Biyu Hou.et al,/,/
- Inhibition of Rac1 ameliorates neuronal oxidative stress damage via reducing Bcl-2/Rac1 complex formation in mitochondria through PI3K/Akt/mTOR Pan Y.et al,Exp Neurol,2017
- Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
- Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing Hyun Jun Park.et al,World J Mens Health,2017
- Fucoidan Protects Dopaminergic Neurons by Enhancing the Mitochondrial Function in a Rotenone-induced Rat Model of Parkinson’s Disease Zhang Li.et al,Aging Dis,2017
- Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats Zhou JC.et al,J Nutr.,2017
- Rescue effects of ginger extract on dose dependent radiation-induced histological and biochemical changes in the kidneys of male Wistar rats Saberi H.et al,Biomed Pharmacother.,2017
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- Effect of intensive endurance training on rat brain and hepatic 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels Mohammad Afzalpour.et al,Journal of Applied Pharmaceutical Science ,2016
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- The effect of prenatal exposure to 900-megahertz electromagnetic field on the, 21-old-day rat testicle Hanc謀 H et al,Reprod Toxicol,2013
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靶點(diǎn)詳情
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最新研究進(jìn)展：8-OHdG是DNA氧化損傷的標(biāo)志物，最新研究發(fā)現(xiàn)，其與肺癌、肝癌、結(jié)直腸癌、卵巢癌等多種癌癥的發(fā)生和發(fā)展有關(guān)。研究人員通過(guò)對(duì)癌癥患者和對(duì)照組進(jìn)行對(duì)比分析，發(fā)現(xiàn)8-OHdG的水平在癌癥患者中明顯升高，因此可以作為腫瘤的生物標(biāo)志物之一。此外，8-OHdG的水平還可以反映環(huán)境暴露和生活方式對(duì)人體DNA的影響。最新研究表明，8-OHdG的水平與吸煙、飲酒、肥胖、缺乏鍛煉等不良生活習(xí)慣有關(guān)。因此，8-OHdG的測(cè)量可以用作評(píng)估生活方式和環(huán)境暴露的生物標(biāo)志物。另外，一些研究還表明，8-OHdG可能與一些神經(jīng)退行性疾病的發(fā)生和發(fā)展有關(guān)，包括帕金森病和阿爾茨海默病。在這些疾病中，氧化應(yīng)激和DNA氧化損傷可能是致病機(jī)制之一，而8-OHdG的測(cè)量可以用作評(píng)估疾病進(jìn)展和治療效果的生物標(biāo)志物。
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