Rabbit Procalcitonin (PCT) ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔降鈣素原(PCT)酶聯免疫試劑盒（CSB-E12875Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PCT含量。PCT即降鈣素原，在健康人群中含量極低。當機體發生細菌感染時，PCT會明顯升高。其研究機制主要是通過檢測它在血液中的水平，輔助醫生判斷是否存在細菌感染、評估感染嚴重程度及指導抗生素的合理使用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，常用于科研領域評估炎癥程度及疾病進展；可為感染性疾病動物模型研究、免疫機制探索及藥物療效評價提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PCT
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-102
1:2	Average %	86
	Range %	81-99
1:4	Average %	95
	Range %	90-103
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of rabbit PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	86-94
EDTA plasma (n=4)	96	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.