Mouse Procalcitonin,PCT ELISA Kit

產(chǎn)品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠降鈣素原(PCT)酶聯(lián)免疫試劑盒（CSB-E10371m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PCT含量。降鈣素原（PCT）是一種蛋白質(zhì)。在健康人體中，PCT 水平極低。當發(fā)生嚴重細菌、真菌、寄生蟲感染及膿毒癥和多臟器功能衰竭時，其水平會顯著升高。研究認為，它可作為炎癥和感染的生物標志物，輔助醫(yī)生判斷病情和指導治療。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，可應用于科研場景中PCT相關機制研究?？蒲腥藛T可將其應用于實驗動物模型中膿毒癥、全身性感染及炎癥性疾病的機制探索，或用于評估藥物干預對炎癥通路的影響。本試劑盒支持多種生物樣本類型檢測，為實驗室開展PCT動態(tài)監(jiān)測、病原體 - 宿主互作研究及免疫調(diào)節(jié)實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

PCT
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	88-93
1:40	Average %	102
	Range %	99-104
1:80	Average %	94
	Range %	89-96
1:160	Average %	97
	Range %	94-101

回收率：

The recovery of mouse PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.