Rabbit Interleukin 1,IL-1 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子白介素1(IL-1)酶聯(lián)免疫試劑盒（CSB-E06896Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1A含量。IL1A即白介素-1α，是一種重要的細(xì)胞因子。其背景在于它在免疫調(diào)節(jié)、炎癥反應(yīng)中扮演關(guān)鍵角色。研究機(jī)制方面，它能激活下游信號通路，誘導(dǎo)炎癥介質(zhì)釋放，參與多種疾病病理過程，如炎癥性疾病、腫瘤等，對其研究有助于疾病治療策略開發(fā)。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)研究中對多組樣本進(jìn)行批量檢測，例如探索炎癥模型動物血清標(biāo)志物變化、評估藥物干預(yù)后組織內(nèi)細(xì)胞因子表達(dá)差異等場景，為實驗室開展免疫調(diào)控機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
縮寫：

IL-1
Uniprot No.：

P04822
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	92
	Range %	85-100
1:4	Average %	95
	Range %	89-101
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of rabbit IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-99
EDTA plasma (n=4)	92	84-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
基因功能參考文獻(xiàn)：
1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
亞細(xì)胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100009250

STRING: 9986.ENSOCUP00000007573

UniGene: Ocu.2080