Rabbit Immunoglobulin E,IgE ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子免疫球蛋白E(IgE)酶聯免疫試劑盒（CSB-E06948Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。IgE即免疫球蛋白E，是介導Ⅰ型超敏反應的抗體，在過敏反應、寄生蟲感染等過程中發揮重要作用。研究機制上，其可與肥大細胞和嗜堿性粒細胞表面受體結合，當變應原再次入侵，結合IgE后促使細胞釋放炎癥介質，引發過敏癥狀。試劑盒檢測范圍為7.8 pg/mL-2000 pg/mL，適用于科研場景中兔模型過敏性炎癥機制探索、寄生蟲感染免疫動態監測、疫苗或藥物干預實驗的療效評估等研究，尤其適合需要高靈敏度檢測微量樣本的應用需求。試劑盒兼容血清與血漿樣本，為體外實驗提供便捷的檢測方案，滿足基礎研究中對免疫指標定量分析的要求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgE
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	91
	Range %	84-96
1:2000	Average %	89
	Range %	82-96
1:4000	Average %	93
	Range %	87-100
1:8000	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	88	85-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.