Mouse Immunoglobulin E,IgE ELISA Kit
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中文名稱：小鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒
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貨號：CSB-E07983m
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規(guī)格：96T/48T
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價格：￥3800/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠免疫球蛋白E(IgE)酶聯(lián)免疫試劑盒（CSB-E07983m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。IgE是介導(dǎo)Ⅰ型超敏反應(yīng)的關(guān)鍵抗體，在過敏反應(yīng)中扮演重要角色。其研究機(jī)制主要圍繞阻斷IgE與其受體結(jié)合，抑制肥大細(xì)胞和嗜堿性粒細(xì)胞活化、脫顆粒，釋放炎癥介質(zhì)，進(jìn)而減輕過敏癥狀，為過敏性疾病治療提供新方向。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中小鼠過敏模型構(gòu)建、免疫調(diào)節(jié)機(jī)制研究、抗過敏藥物開發(fā)等場景，特別在評估實(shí)驗(yàn)動物體內(nèi)IgE水平變化、免疫應(yīng)答動態(tài)監(jiān)測等實(shí)驗(yàn)中具有高特異性和穩(wěn)定性。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：IgE
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma
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檢測范圍：1.56 ng/mL-100 ng/mL
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靈敏度：0.39 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 89-95 1:2 Average % 102 Range % 99-105 1:4 Average % 93 Range % 87-96 1:8 Average % 99 Range % 94-101 -
回收率：
The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 91-97 EDTA plasma (n=4) 95 91-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 100 2.435 2.328 2.382 2.245 50 1.704 1.684 1.694 1.557 25 1.182 1.166 1.174 1.037 12.5 0.577 0.590 0.584 0.447 6.25 0.400 0.410 0.405 0.268 3.12 0.307 0.292 0.300 0.163 1.56 0.258 0.248 0.253 0.116 0 0.138 0.136 0.137 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Multi-omics analysis reveals the alleviating effects of Bifidobacterium infantis M-16V on food allergy through modulation of gut microbiota, metabolome, and gene expression in mice and infants N Li, Y Zhang, S Gao, Q Zhang, X Wang, W Cui, C Xu,Microbiome,2024
- Hydrogen Gas Inhalation Alleviates Airway Inflammation and Oxidative Stress on Ovalbumin-Induced Asthmatic BALB/c Mouse Model W He, MH Rahman, J Bajgai, S Abdul-Nasir, C Mo,Antioxidants,2024
- Иммунная составляющая патогенетической терапии атопического дерматита ВД Боковой, МА Десятова, АВ Коротков,/,2024
- Bongkrekic acid alleviates airway inflammation via breaking the mPTP/mtDAMPs/RAGE feedback loop in a steroid-insensitive asthma model Y Chen,Biomedicine & pharmacotherapy,2024
- Double Emulsion‐Mediated Delivery of Polyphenol Mixture Alleviates Atopic Dermatitis S Choi,Advanced healthcare materials,2023
- Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin H Wan,Annals of translational medicine,2023
- RAGE mediates airway inflammation via the HDAC1 pathway in a toluene diisocyanate-induced murine asthma model X Peng,BMC pulmonary medicine,2023
- Food Bioscience W Cui,/,2023
- Anti-allergic function of the cell wall (DeinoWall) from Deinococcus radiodurans F Chen,Molecular immunology,2022
- Identification of histone acetylation in a murine model of allergic asthma by proteomic analysis Y Ren,Experimental biology and medicine,2020
- Enhancement of Anti-allergic Effect of Diethylcarbamazine Citrate in Asthmatic Mouse Model: Testing of Anti-drug Antibodies and Quercetin N Abdul-Razek,Iranian Journal of Allergy, Asthma and Immunology (Bimonthly),2020
- Baicalin regulates Treg/Th17 cell imbalance by inhibiting autophagy in allergic rhinitis J Li,Molecular Immunology,2020
- Immunomodulatory effects of two silymarin isomers in a Balb/c mouse model of allergic asthma EM Nasab,Allergol Immunopathol (Madr),2020
- Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2 W Wang,Med. Sci. Monit,2020
- Long noncoding RNA atlas of the inflammation caused by asthma in mice C Zhang,Archives of Pharmacal Research,2020
- Licorice (Glycyrrhizza glabra) Extract Prevents Production of Th2 Cytokines and Free Radicals Induced by Ova Albumin in Mice Hala AbdEl-Rahman Hassan Khattab.et al,International Journal of Pharmacology,2018
- Glycyrrhizin ameliorates atopic dermatitis-like symptoms through inhibition of HMGB1 Ying Wang.et al,International Immunopharmacology,2018
- Fine particulate matter aggravates allergic airway inflammation through thymic stromal lymphopoietin activation in mice Liu Y.et al,Mol Med Rep. ,2017
- Anti-inflammatory Effect of Grape Seed Extract on Bronchial Asthma Induced in Mice Hashemi J M.et al,Life Science Journal,2016
- Chrysin alleviates allergic inflammation and airway remodeling in a murine model of chronic asthma Yao J. et al,Int Immunopharmacol,2016
- Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3 Deng YQ. et al,PLoS One,2015
產(chǎn)品評價
平均分:
樣品類型：血清
樣品信息：小鼠
稀釋比：沒有稀釋
產(chǎn)品評價： 我用ige的elisa試劑盒檢測小鼠血清樣本，OD值梯度值很好，計算的標(biāo)準(zhǔn)曲線擬合度也很好，實(shí)驗(yàn)結(jié)果濃度值與小鼠HE結(jié)果高度吻合，實(shí)驗(yàn)結(jié)果可靠，不愧是國產(chǎn)大牌，下次會繼續(xù)購買。
By 李老師
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