Pig fibroblast growth factor 19(FGF19)ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬成纖維細胞生長因子19(FGF19)酶聯免疫試劑盒（CSB-E17583p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FGF19含量。FGF19是一種成纖維細胞生長因子。它在調節膽汁酸合成、糖脂代謝等方面有重要作用。研究機制上，FGF19結合并激活FGFR4-βKlotho復合物，啟動下游信號通路，調控基因表達。相關研究為代謝性疾病等治療提供潛在靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于豬模型中代謝調控機制研究、肝臟疾病模型構建、營養干預實驗等科研場景，可為探究FGF19在能量平衡、腸道功能及病理生理過程中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

FGF19
Uniprot No.：

F1RY79
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FGF19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-94
1:2	Average %	99
	Range %	91-107
1:4	Average %	96
	Range %	92-105
1:8	Average %	96
	Range %	88-98

回收率：

The recovery of pig FGF19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.