Human bovine serum albumin (BSA) ELISA kit
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中文名稱(chēng)：人的牛血清白蛋白(BSA)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E08633h
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人的牛血清白蛋白(BSA)酶聯(lián)免疫試劑盒（CSB-E08633h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的BSA含量。BSA即牛血清白蛋白，背景上，它來(lái)源豐富、價(jià)格低廉，在生物醫(yī)學(xué)領(lǐng)域廣泛應(yīng)用。研究機(jī)制方面，其可作為載體蛋白，結(jié)合藥物、小分子等；也能模擬體內(nèi)血漿環(huán)境，用于藥物體外研究，還可用于酶活性測(cè)定、免疫分析等。試劑盒檢測(cè)范圍為0.312 μg/mL-80 μg/mL，靈敏度為0.312 μg/mL。本試劑盒通過(guò)預(yù)包被特異性抗體，利用樣本中BSA與酶標(biāo)記BSA競(jìng)爭(zhēng)結(jié)合抗體的特性，結(jié)合顯色反應(yīng)實(shí)現(xiàn)定量分析，可廣泛用于實(shí)驗(yàn)室評(píng)估樣本制備過(guò)程中BSA的添加濃度、檢測(cè)實(shí)驗(yàn)體系中的外源性BSA干擾，或作為生物制品生產(chǎn)過(guò)程中蛋白成分的質(zhì)控工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：BSA
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種屬：Homo sapiens (Human)
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樣本類(lèi)型：serum, plasma
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檢測(cè)范圍：0.312 μg/mL-80 μg/mL
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靈敏度：0.312 μg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 80-92 1:2 Average % 95 Range % 91-105 1:4 Average % 95 Range % 92-100 1:8 Average % 93 Range % 85-98 -
回收率：
The recovery of human BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-108 EDTA plasma (n=4) 97 90-110 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average 80 0.286 0.291 0.289 20 0.435 0.442 0.439 5 0.589 0.593 0.591 1.25 0.799 0.805 0.802 0.312 1.228 1.237 1.233 0 1.772 1.782 1.777 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- An electrochemical aptasensing platform for carbohydrate antigen 125 based on the use of flower-like gold nanostructures and target-triggered strand displacement amplification Chen J,Microchimica Acta,2019
- Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification Qingzhen Zhou.et al,Royal Society of Chemistry,2018
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