Chicken rudimental bovine serum albumin(BSA) check-up ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞的牛血清白蛋白(BSA) 殘留檢測酶聯免疫試劑盒（CSB-EQ027303CH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BSA含量。試劑盒檢測范圍為0.312 μg/mL-80 μg/mL，靈敏度為0.312 μg/mL。適用于科研領域中對生物制品、疫苗生產或實驗樣本處理流程中BSA污染物的質量控制；適用于評估樣本純化工藝效果、細胞培養基質殘留分析或生物制藥流程中的BSA監控；助力科研人員優化實驗體系并保障數據可靠性本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

BSA
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-80 μg/mL
靈敏度：

0.312 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	106
	Range %	100-112
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of chicken BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.