Human Interleukin-31 receptor subunit alpha(IL31RA) ELISA kit

說明書下載

中文名稱：

人白介素-31受體α亞基(IL31RA)酶聯免疫試劑盒
貨號：

CSB-EL011654HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人白介素-31受體α亞基(IL31RA)酶聯免疫試劑盒（CSB-EL011654HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL31RA含量。IL31RA是一種重要靶點。它參與多種生理和病理過程，尤其在免疫調節與炎癥反應方面。其研究機制主要聚焦于它與配體IL-31結合后激活下游信號通路，影響細胞的生物學行為，對相關疾病的發生發展意義重大，是藥物研發的潛在方向。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，本試劑盒基于高特異性配對抗體開發，操作流程標準化，適用于科研領域中對IL - 31信號通路的機制研究、疾病模型構建或藥物干預效果評估，可為探索IL31RA在免疫調節、炎癥反應或皮膚相關疾病中的功能提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Class I cytokine receptor ELISA Kit; CRL 3 ELISA Kit; CRL ELISA Kit; CRL3 ELISA Kit; Cytokine receptor like 3 ELISA Kit; Cytokine receptor-like 3 ELISA Kit; GLM R ELISA Kit; GLM-R ELISA Kit; GLMR ELISA Kit; Gp130 like monocyte receptor ELISA Kit; GP130 like receptor ELISA Kit; Gp130-like monocyte receptor ELISA Kit; Gp130-like receptor ELISA Kit; GPL ELISA Kit; HGLM R ELISA Kit; HGLM-R ELISA Kit; HGLMR ELISA Kit; IL 31 receptor subunit alpha ELISA Kit; IL 31R subunit alpha ELISA Kit; IL 31RA ELISA Kit; IL-31 receptor subunit alpha ELISA Kit; IL-31R subunit alpha ELISA Kit; IL-31R-alpha ELISA Kit; IL-31RA ELISA Kit; IL31 receptor subunit alpha ELISA Kit; IL31R subunit alpha ELISA Kit; IL31R_HUMAN ELISA Kit; IL31RA ELISA Kit; Interleukin 31 receptor A ELISA Kit; interleukin 31 receptor subunit alpha ELISA Kit; Interleukin-31 receptor subunit alpha ELISA Kit; MGC125346 ELISA Kit; PLCA 2 ELISA Kit; PLCA2 ELISA Kit; PRO21384 ELISA Kit; Soluble type I cytokine receptor CRL3 ELISA Kit; ZcytoR17 ELISA Kit
縮寫：

IL31RA
Uniprot No.：

Q8NI17
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL31RA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	89-99
1:10	Average %	102
	Range %	96-107
1:20	Average %	96
	Range %	92-103
1:40	Average %	89
	Range %	82-94

回收率：

The recovery of human IL31RA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-91
EDTA plasma (n=4)	97	92-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.943	2.875	2.909	2.819
200	2.387	2.246	2.317	2.227
100	1.554	1.512	1.533	1.443
50	0.826	0.808	0.817	0.727
25	0.467	0.455	0.461	0.371
12.5	0.259	0.246	0.253	0.163
6.25	0.179	0.173	0.176	0.086
0	0.091	0.089	0.090

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Mediates IL31-induced itch, probably in a manner dependent on cation channels TRPA1 and TRPV1. Positively regulates numbers and cycling status of immature subsets of myeloid progenitor cells in bone marrow in vivo and enhances myeloid progenitor cell survival in vitro.
基因功能參考文獻：
1. The first demonstration of the involvement of the IL-31/IL-31R axis in cancer came from studies in patients with mycosis fungoides/Sezary syndrome, the most frequent, cutaneous T cell lymphoma. Tumor cells were shown to produce IL-31, whose serum levels correlated with pruritus intensity. PMID: 28408397
2. The interleukin IL-31/IL-31receptor axis contributes to tumor growth in human follicular lymphoma. PMID: 25283844
3. detected in keratinocytes and nerve fibers in the dermis of atopic dermatitis and in the neurons of normal dorsal root ganglia PMID: 24667097
4. IL31RA is a functional receptor expressed by a small subpopulation of IL-31RA(+)/TRPV1(+)/TRPA1(+) neurons and is a critical neuroimmune link between TH2 cells and sensory nerves for the generation of T cell-mediated itch. PMID: 24373353
5. IL-31 and IL-31RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. PMID: 23392388
6. IFN-gamma-induced IL-31RA upregulation in dermal microvascular endothelium is processed via JNK and PI3 kinase activation PMID: 20849534
7. The identification of OSMR and IL31RA gene pathology provides an explanation of the high prevalence of primary cutaneous amyloidosis in Taiwan as well as new insight into disease pathophysiology. PMID: 19690585
8. A novel type I cytokine receptor is expressed on monocytes, signals proliferation, and activates STAT-3 and STAT-5. PMID: 11877449
9. GPL is a novel cytokine receptor related to GP130 and leukemia inhibitory factor receptor PMID: 14504285
10. the molecular mechanisms underlying GPL-mediated signal transduction PMID: 15194700
11. IL-31 receptor alpha expression in epidermal keratinocytes is modulated by cell differentiation and interferon gamma PMID: 18580959
12. ). Binding of IL-31 to its receptor activates Jak/STAT, PI3K/AKT and MAPK pathways.[review] PMID: 18926762
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相關疾?。?/div>
Amyloidosis, primary localized cutaneous, 2 (PLCA2)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, presynaptic cell membrane. Cell projection, axon.
蛋白家族：

Type I cytokine receptor family, Type 2 subfamily
組織特異性：

Expressed in CD14- and CD56-positive blood cells. Expressed in macrophages. Expressed in keratinocytes. Expressed in a subset of dorsal root ganglia neurons (at protein level). Expressed at low levels in testis, ovary, brain, prostate, placenta, thymus, b
數據庫鏈接：

HGNC: 18969

OMIM: 609510

KEGG: hsa:133396

STRING: 9606.ENSP00000415900

UniGene: Hs.55378

Human Interleukin-31 receptor subunit alpha(IL31RA) ELISA kit

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