Human Beta-Endorphin,β-EP ELISA Kit

Human Beta-Endorphin,β-EP ELISA Kit

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中文名稱：

人β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒
貨號：

CSB-E06821h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E06821h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、腦脊液（CSF）、唾液、組織勻漿樣本中的β-EP含量。β-EP（β-內(nèi)啡肽）是體內(nèi)天然阿片肽，在痛覺調(diào)節(jié)、情緒調(diào)控等方面有重要作用。研究機制上，其能與體內(nèi)阿片受體結(jié)合，產(chǎn)生鎮(zhèn)痛、愉悅感等效果。對它的研究有助于深入了解疼痛、成癮及情緒相關(guān)疾病發(fā)病機制與治療。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于神經(jīng)生物學(xué)、疼痛機制及應(yīng)激反應(yīng)等基礎(chǔ)研究領(lǐng)域；其水平變化與神經(jīng)退行性疾病、慢性疼痛綜合征及精神類疾病的研究密切相關(guān)；支持科研人員對中樞神經(jīng)系統(tǒng)、內(nèi)分泌系統(tǒng)及相關(guān)病理模型的深入分析；樣本兼容性涵蓋體液與組織裂解液，特別適用于跨樣本類型的對比研究，例如腦脊液與血清中β-EP的動態(tài)關(guān)聯(lián)分析，或特定腦區(qū)組織勻漿的局部濃度檢測；為探索β-EP在神經(jīng)信號傳導(dǎo)、應(yīng)激適應(yīng)機制及藥物干預(yù)效應(yīng)等方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Homo sapiens (Human)
樣本類型：

serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	99
	Range %	91-110
1:4	Average %	97
	Range %	92-105
1:8	Average %	93
	Range %	86-98

回收率：

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.537	2.568	2.553	2.443
2000	2.010	1.999	2.005	1.895
1000	1.600	1.532	1.566	1.456
500	1.032	1.122	1.077	0.967
250	0.567	0.600	0.584	0.474
125	0.391	0.410	0.401	0.291
62.5	0.242	0.254	0.248	0.138
0	0.109	0.111	0.110

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Measuring of serum taurine and beta endorphin in women as a pre-early marker for diagnosis of cancer breast SA Dardier,/,2023
Effects of circuit-type resistance training on industrial opiate induced neurotransmitter disruption and performance changes of previously addicted men H Arazi,Biomedical Human Kinetics,2020
In major depression, increased kappa and mu opioid receptor levels are associated with immune activation Al-Hakeim H K, et al,Acta Neuropsychiatrica,2019
In Major Depression, Increased Serum Dynorphin and Kappa Opioid Receptor Levels are Positively Associated with Mu Opioid Receptor Levels and Immune Activation and Are Attenuated by Nicotine Dependence Hussein Al-Hakeim, et al,Preprints,2019
Development of a Novel Neuro-Immune and Opioid-Associated Fingerprint with a Cross-Validated Ability to Identify and Authenticate Unknown Patients with Major Depression: Far Beyond Differentiation, Discrimination and Classification Hussein Al-Hakeim, et al,Preprints,2019
IL-10 is associated with increased mu-opioid receptor levels in major depressive disorder Al-Fadhel SZ, et al,European Psychiatry,2019
Sweating away depression? The impact of intensive exercise on depression. Balchin R.et al,J Affect Disord.,2016
Hypomagnetic field alters circadian rhythm and increases algesia in adult male mice MO Wei-Chuan. et al,Progress in Biochemistry and Biophysics,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.