Mouse Beta-Endorphin,β-EP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠β內(nèi)啡肽(β-EP)酶聯(lián)免疫試劑盒（CSB-E06827m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的β-EP含量。β-EP即β-內(nèi)啡肽，是內(nèi)源性阿片肽家族中的重要成員，廣泛分布于中樞神經(jīng)系統(tǒng)和外周組織。其研究機(jī)制主要圍繞它與阿片受體結(jié)合，在痛覺調(diào)制、神經(jīng)內(nèi)分泌調(diào)節(jié)、情緒調(diào)控等方面發(fā)揮關(guān)鍵作用，對了解機(jī)體生理和病理過程意義重大。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，適用于基礎(chǔ)科研領(lǐng)域，如探究中樞神經(jīng)系統(tǒng)疾病模型、應(yīng)激反應(yīng)機(jī)制或鎮(zhèn)痛藥物研究中β-EP的動態(tài)變化，亦可用于評估動物模型中組織特異性β-EP表達(dá)水平。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

beta-Endorphin, beta-EP
縮寫：

β-EP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.195 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	95
	Range %	91-98
1:4	Average %	101
	Range %	97-104
1:8	Average %	92
	Range %	89-95

回收率：

The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	94	90-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.