Human B-cell differentiation antigen CD72(CD72) ELISA kit

文獻引用 (1) 說明書下載

中文名稱：

人B細胞分化蛋白CD72(CD72)酶聯免疫試劑盒
貨號：

CSB-EL004955HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人B細胞分化蛋白CD72(CD72)酶聯免疫試劑盒（CSB-EL004955HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD72含量。CD72是重要靶點，它是B細胞表面糖蛋白，在B細胞發育、活化及免疫調節中起關鍵作用。研究機制方面，聚焦于其與配體相互作用，通過信號通路影響B細胞功能，針對它的研究有望為免疫相關疾病如自身免疫病、腫瘤等治療帶來新策略。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于科研領域探究B細胞相關免疫機制，如系統性紅斑狼瘡、類風濕性關節炎等自身免疫疾病模型中的B細胞功能研究，或腫瘤微環境中B淋巴細胞活化狀態的分子機制分析，為免疫調控網絡研究提供定量檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72
縮寫：

CD72
Uniprot No.：

P21854
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.225 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-103
1:2	Average %	91
	Range %	86-98
1:4	Average %	92
	Range %	88-97
1:8	Average %	101
	Range %	96-15

回收率：

The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	103-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.588	2.787	2.688	2.535
30	2.169	2.236	2.203	2.050
15	1.674	1.744	1.709	1.556
7.5	1.322	1.223	1.273	1.120
3.75	0.752	0.744	0.748	0.595
1.8	0.363	0.375	0.369	0.216
0.9	0.261	0.255	0.258	0.105
0	0.149	0.156	0.153

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

A Predictive Model for Initial Platinum-Based Chemotherapy Efficacy in Patients with Postoperative Epithelial Ovarian Cancer Using Tissue-Derived Small Extracellular Vesicles S Shen, C Wang, J Gu, F Song, X Wu,Journal of Extracellular Vesicles,2024

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功能：

Plays a role in B-cell proliferation and differentiation.
基因功能參考文獻：
1. Interferon-alpha-induced CD100 expression on naive CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction. PMID: 28222623
2. Increased soluble CD72 in systemic lupus erythematosus is in association with disease activity and lupus nephritis PMID: 26883681
3. CD72 expression on activated B cells of SLE patients was significantly lower than that of normal controls. sema3A enhanced CD72 expression of B cells, but it was still lower in SLE patients than in normal individuals. PMID: 24461079
4. data demonstrated aberrant expression of CD72 exists on B cells of myasthenia gravis and multiple sclerosis patients and expression level of CD72 molecule has a significantly negative correlation with anti-AchR antibody levels in myasthenia gravis PMID: 23184497
5. regulates serum immunoglobulin leveln and disease resistance in systemic lupus erythematosus PMID: 23268649
6. CD72 mRNA expression level correlates with Sema4D expression in peripheral blood mononuclear cells in immune thrombocytopenia. PMID: 22111667
7. CD100, CD72 and CD45 were expressed in placenta and exhibited different mRNA and protein levels in normal pregnancy and miscarriage. Protein levels were highly dysregulated around 10 weeks of gestation in first and second miscarriage placentas. PMID: 22606231
8. CD100-CD72 interaction can be the mechanism by which NK cell communicate with B cells. PMID: 17786190
9. Data show that ligation of CD72 with the BU40, or with rCD100 negatively regulates KIT-mediated mast cell proliferation, chemotaxis, and chemokine production. PMID: 20100931
10. results indicated that the presence of CD72-*2 allele decreases risk for human systemic lupus erythematosus conferred by FCGR2B-232Thr, possibly by increasing the AS isoform of CD72 PMID: 15459183
11. CD72 is a key molecule in regulating mature B cell differentiation; CD72 signaling reduces the expression of X-box binding protein 1 in B cells PMID: 16047337
12. increased nucleotide mutation of CD72 mRNA accounts for the decreased expression level of CD72 in B cells, and it might be related to hyperactivity of B cells in patients with SLE PMID: 17121583
13. CD72 polymorphisms are associated with age of appearing clinical manifestations in idiopathic thrombocytopenic purpura in Chinese patients. PMID: 18071878
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亞細胞定位：

Membrane; Single-pass type II membrane protein.
組織特異性：

Pre-B-cells and B-cells but not terminally differentiated plasma cells.
數據庫鏈接：

HGNC: 1696

OMIM: 107272

KEGG: hsa:971

STRING: 9606.ENSP00000259633

UniGene: Hs.116481

Human B-cell differentiation antigen CD72(CD72) ELISA kit

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