Horse Hepcidin(HAMP) ELISA kit

說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

馬鐵調(diào)素(HAMP)酶聯(lián)免疫試劑盒（CSB-EL010124HO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的HAMP含量。試劑盒檢測(cè)范圍為7.8 ng/mL-500 ng/mL，適用于科研領(lǐng)域中對(duì)鐵代謝調(diào)控機(jī)制的研究，如探究貧血、鐵代謝紊亂疾病的分子機(jī)制，評(píng)估動(dòng)物模型或細(xì)胞實(shí)驗(yàn)中鐵調(diào)素表達(dá)水平的變化，以及相關(guān)藥物干預(yù)對(duì)鐵穩(wěn)態(tài)影響的體外分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide|putative liver tumor regressor
縮寫(xiě)：

HAMP
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of horse HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.