Pig Hepcidin(HAMP) ELISA kit

Pig Hepcidin(HAMP) ELISA kit

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文獻引用 (1) 說明書下載

中文名稱：

豬鐵調素(HAMP)酶聯免疫試劑盒
貨號：

CSB-EL010124PI
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬鐵調素(HAMP)酶聯免疫試劑盒（CSB-EL010124PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HAMP含量。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，可為豬鐵代謝機制研究、營養性缺鐵模型構建、疾病狀態下鐵調素動態變化分析等科研項目提供可靠工具，尤其適用于農業科學、獸醫學領域中對鐵穩態調控的分子機制探索及生物標志物篩選。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

HAMP ELISA Kit; Hepcidin ELISA Kit
縮寫：

HAMP
Uniprot No.：

Q8MJ80
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.38 ng/mL-600 ng/mL
靈敏度：

2.34 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-109
1:2	Average %	94
	Range %	89-99
1:4	Average %	98
	Range %	93-103
1:8	Average %	89
	Range %	84-95

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

600

2.751

2.634

2.692

2.543

300

2.164

2.267

2.215

2.066

150

1.424

1.368

1.396

1.247

0.859

0.829

0.844

0.695

37.5

0.428

0.421

0.424

0.275

18.75

0.283

0.273

0.278

0.129

9.38

0.201

0.194

0.198

0.049

0.153

0.145

0.149

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Comparison of Oral and Parenteral Iron Administration on Iron Homeostasis, Oxidative and Immune Status in Anemic Neonatal Pigs Dong Z, et al,Biological Trace Element Research,2019

功能：

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
基因功能參考文獻：
1. this study demonstrates that urine hepcidin-25 concentrations strongly correlate with hepatic hepcidin mRNA abundance, plasma hepcidin-25 levels, iron transferrin saturation and non-heme liver iron levels. PMID: 26323096
2. Data suugest that hepcidin might had antiinflammatory function and is a candidate regulator of the cross-talk between iron regulation and inflammation. PMID: 25774043
3. report the full-length cDNA sequences of porcine hepcidin and liver-expressed antimicrobial peptide-2 (LEAP-2) PMID: 16051358
亞細胞定位：

Secreted.
蛋白家族：

Hepcidin family
數據庫鏈接：

KEGG: ssc:397207

STRING: 9823.ENSSSCP00000028422

UniGene: Ssc.376

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.