Dog S100 calcium binding protein B (S100B) ELISA kit

產品詳情

產品描述：

犬S100B蛋白(S100B)酶聯免疫試劑盒（CSB-EL020643DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的S100B含量。S100B是一種鈣結合蛋白，主要存在于神經膠質細胞中。在神經系統疾病如阿爾茨海默病、腦損傷中，S100B水平會出現異常。研究發現它可通過多種信號通路影響神經細胞生長、凋亡等，其異常表達可能參與疾病的發生發展。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，該產品適用于神經科學基礎研究、疾病模型評估及藥物開發等科研場景，例如通過檢測外周血或腦組織樣本中的S100B水平，探究犬類神經退行性病變機制或創傷性腦損傷的生物標志物動態變化，為動物實驗提供可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

S100B
Uniprot No.：

E2QTP3
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	93
	Range %	87-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	98
	Range %	94-103

回收率：

The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	89	84-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.