ZBTB7A

ZBTB7A，全稱為鋅指和BTB結(jié)構(gòu)域包含蛋白7A，是一種含有N端BTB/POZ結(jié)構(gòu)域和C端鋅指結(jié)構(gòu)域的轉(zhuǎn)錄因子。該蛋白在多種細(xì)胞類型中表達(dá)，參與基因調(diào)控，影響細(xì)胞增殖、分化和凋亡等過程。ZBTB7A蛋白通過其鋅指結(jié)構(gòu)域識(shí)別特定的DNA序列，結(jié)合到靶基因的啟動(dòng)子區(qū)域，從而調(diào)控基因表達(dá)。研究表明，ZBTB7A蛋白在多種生理和病理過程中發(fā)揮重要作用，包括細(xì)胞周期調(diào)控、細(xì)胞分化、細(xì)胞凋亡等。ZBTB7A蛋白的表達(dá)異常與多種疾病相關(guān)，如癌癥、免疫疾病和神經(jīng)系統(tǒng)疾病等。針對(duì)ZBTB7A蛋白的藥物研發(fā)正在積極進(jìn)行中，包括小分子抑制劑、RNA干擾技術(shù)和基因編輯技術(shù)等。這些藥物有望通過調(diào)節(jié)ZBTB7A蛋白的表達(dá)或活性，從而治療相關(guān)疾病。

熱銷產(chǎn)品

ZBTB7A Recombinant Monoclonal Antibody (CSB-RA063930A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: A431 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HeLa whole cell lysate(30μg), A549 whole cell lysate(30μg)
All lanes: ZBTB7A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 61 kDa
Observed band size: 70 kDa
Exposure time：2min

IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of MCF-7 cell with CSB-RA063930A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing BxPC-3 cells stained with CSB-RA063930A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

ZBTB7A Antibody

ZBTB7A Protein

ZBTB7A Antibodies

ZBTB7A for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA026343GA01HU	ZBTB7A Antibody	Human,Mouse	ELISA,WB,IHC
CSB-RA063930A0HU	ZBTB7A Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC

ZBTB7A Proteins

ZBTB7A Proteins for Homo sapiens (Human)

產(chǎn)品貨號(hào)	產(chǎn)品名稱	來源
CSB-YP026343HU CSB-EP026343HU CSB-BP026343HU CSB-MP026343HU CSB-EP026343HU-B	Recombinant Human Zinc finger and BTB domain-containing protein 7A (ZBTB7A)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli