USP7
USP7，即泛素特異性肽酶7，別名TEF1、HAUSP、HAFOUS，是一種去泛素化酶，屬于肽酶C19家族，其中包括泛素水解酶。
USP7可通過去泛素化目標(biāo)蛋白質(zhì)，如p53（一種腫瘤抑制蛋白）和WASH（內(nèi)體蛋白質(zhì)回收所必需的），調(diào)節(jié)它們的活動(dòng)，這通過抵消參與相應(yīng)過程的蛋白質(zhì)（如HDM2和TRIM27）的相反泛素連接酶活性來實(shí)現(xiàn)。
USP7在細(xì)胞周期、DNA修復(fù)、染色質(zhì)重塑和表觀遺傳調(diào)控中扮演關(guān)鍵角色。異常激活或過表達(dá)的USP7可能促進(jìn)腫瘤發(fā)生和病毒性疾病，使其成為治療干預(yù)的靶點(diǎn)。USP7還與多種疾病相關(guān)，包括神經(jīng)發(fā)育障礙等。
熱銷產(chǎn)品
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7),Partial (CSB-EP849973HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
USP7 Recombinant Monoclonal Antibody (CSB-RA192026A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: HAUSP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 129, 127 kDa
Observed band size: 140 kDa
IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA192026A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
USP7 Antibodies
USP7 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA849973LA01HU | USP7 Antibody | Human | ELISA, IHC, IF |
| CSB-PA849973LB01HU | USP7 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA849973LC01HU | USP7 Antibody, FITC conjugated | Human | |
| CSB-PA849973LD01HU | USP7 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA192026A0HU | USP7 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF |
USP7 Proteins
USP7 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP700351RA CSB-EP700351RA CSB-BP700351RA CSB-MP700351RA CSB-EP700351RA-B |
Recombinant Rat Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
USP7 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP721329MO CSB-EP721329MO CSB-BP721329MO CSB-MP721329MO CSB-EP721329MO-B |
Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
USP7 Proteins for Gallus gallus (Chicken)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP764911CH CSB-EP764911CH CSB-BP764911CH CSB-MP764911CH CSB-EP764911CH-B |
Recombinant Chicken Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
USP7 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP849973HU CSB-BP849973HU CSB-MP849973HU CSB-EP849973HU-B |
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP849973HU | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7),Partial | E.coli |
| CSB-EP849973HU1 | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial | E.coli |
USP7 Proteins for Drosophila melanogaster (Fruit fly)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP894292DLU CSB-EP894292DLU CSB-BP894292DLU CSB-MP894292DLU CSB-EP894292DLU-B |
Recombinant Drosophila melanogaster Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
