USP4
USP4，即泛素特異性蛋白酶4，也稱為UNP、UNPH。USP4由人類USP4基因編碼，該基因定位于染色體3的3p21.31區(qū)域。USP4是一種去泛素化酶，屬于半胱氨酸蛋白酶家族，能夠從目標(biāo)蛋白質(zhì)上去除泛素化修飾，調(diào)節(jié)蛋白質(zhì)的穩(wěn)定性和功能。
USP4含有DUSP-UBL結(jié)構(gòu)域和一個(gè)UBL插入催化結(jié)構(gòu)域，與其同源蛋白USP11和USP15共享相同的結(jié)構(gòu)域組織。USP4在多種細(xì)胞和生物學(xué)過(guò)程中發(fā)揮關(guān)鍵作用，包括維持內(nèi)質(zhì)網(wǎng)的操作保真度、調(diào)節(jié)細(xì)胞增殖和發(fā)育以及固有免疫的關(guān)鍵途徑。當(dāng)USP4的表達(dá)或活性異常時(shí)，它與多種病理狀態(tài)的進(jìn)展有關(guān)，尤其是癌癥。
熱銷產(chǎn)品
USP4 Antibody (CSB-PA614393LA01HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: USP4 antibody at 6.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 109, 104, 36 kDa
Observed band size: 109 kDa
IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-PA614393LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
USP4 Antibodies
USP4 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA614393LA01HU | USP4 Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA614393LB01HU | USP4 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA614393LC01HU | USP4 Antibody, FITC conjugated | Human | |
| CSB-PA614393LD01HU | USP4 Antibody, Biotin conjugated | Human | ELISA |
USP4 Proteins
USP4 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP614393HU CSB-EP614393HU CSB-BP614393HU CSB-MP614393HU CSB-EP614393HU-B |
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 4 (USP4), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
