TSPO
TSPO（轉(zhuǎn)運(yùn)蛋白）全稱為線粒體外膜轉(zhuǎn)位蛋白，也被稱為外周型苯二氮?受體或18 kDa線粒體膜蛋白。TSPO廣泛分布于神經(jīng)、免疫及內(nèi)分泌系統(tǒng)中，主要參與膽固醇跨線粒體膜運(yùn)輸，調(diào)控類固醇激素合成，并與線粒體功能、細(xì)胞凋亡密切相關(guān)。TSPO表達(dá)異常與阿爾茨海默病、帕金森病等神經(jīng)退行性疾病相關(guān)，在腦損傷區(qū)域或腫瘤組織中常出現(xiàn)高表達(dá)現(xiàn)象。近年來TSPO作為生物標(biāo)志物被用于神經(jīng)炎癥和膠質(zhì)瘤的PET影像診斷，其特異性配體PK11195已在臨床中應(yīng)用。針對(duì)焦慮癥和創(chuàng)傷后應(yīng)激障礙，以TSPO為靶點(diǎn)的藥物如埃替拉侖已進(jìn)入II期臨床試驗(yàn)，顯示出調(diào)節(jié)神經(jīng)類固醇合成的潛力。在癌癥領(lǐng)域，靶向TSPO的藥物可通過誘導(dǎo)腫瘤細(xì)胞線粒體膜電位崩潰促進(jìn)凋亡，目前多項(xiàng)基于TSPO調(diào)控的抗癌方案正在臨床前驗(yàn)證階段。
熱銷產(chǎn)品
TSPO Recombinant Monoclonal Antibody (CSB-RA025168A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue
All lanes: PBR antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 19, 11 KDa
Observed band size: 19 KDa
IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA025168A0HU diluted at 1:117 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC3 cells with CSB-RA025168A0HU at 1:39, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating PTGS2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA025168A0HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA025168A0HU (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
Overlay histogram showing HepG2 cells stained with CSB-RA025168A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
TSPO Antibodies
TSPO for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA050257 | TSPO Antibody | Human | WB, ELISA |
| CSB-PA536576LA01HU | TSPO Antibody | Human | ELISA, IHC |
| CSB-RA025168A0HU | TSPO Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB, IHC, IF, FC, IP |
TSPO Proteins
TSPO Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP025168RA1 CSB-EP025168RA1 CSB-BP025168RA1 CSB-MP025168RA1 CSB-EP025168RA1-B |
Recombinant Rat Translocator protein (Tspo), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF025168RA | Recombinant Rat Translocator protein (Tspo) | in vitro E.coli expression system |
TSPO Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP025168BO1 CSB-EP025168BO1 CSB-BP025168BO1 CSB-MP025168BO1 CSB-EP025168BO1-B |
Recombinant Bovine Translocator protein (TSPO), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF025168BO | Recombinant Bovine Translocator protein (TSPO) | in vitro E.coli expression system |
TSPO Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP025168HU1 CSB-EP025168HU1 CSB-BP025168HU1 CSB-MP025168HU1 CSB-EP025168HU1-B |
Recombinant Human Translocator protein (TSPO), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-YP536576HU CSB-EP536576HU CSB-BP536576HU CSB-MP536576HU CSB-EP536576HU-B |
Recombinant Human Putative peripheral benzodiazepine receptor-related protein (TSPO) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF025168HU | Recombinant Human Translocator protein (TSPO) | in vitro E.coli expression system |
TSPO Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP025168MO1 CSB-EP025168MO1 CSB-BP025168MO1 CSB-MP025168MO1 CSB-EP025168MO1-B |
Recombinant Mouse Translocator protein (Tspo), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF025168MO | Recombinant Mouse Translocator protein (Tspo) | in vitro E.coli expression system |
| CSB-MP025168MO | Recombinant Mouse Translocator protein (Tspo)-VLPs | Mammalian cell |
TSPO Proteins for Arabidopsis thaliana (Mouse-ear cress)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP526860DOA1 CSB-EP526860DOA1 CSB-BP526860DOA1 CSB-MP526860DOA1 CSB-EP526860DOA1-B |
Recombinant Arabidopsis thaliana Translocator protein homolog (TSPO), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF526860DOA | Recombinant Arabidopsis thaliana Translocator protein homolog (TSPO) | in vitro E.coli expression system |
TSPO Proteins for Sus scrofa (Pig)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP764926PI1 CSB-EP764926PI1 CSB-BP764926PI1 CSB-MP764926PI1 CSB-EP764926PI1-B |
Recombinant Pig Translocator protein (TSPO), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF764926PI | Recombinant Pig Translocator protein (TSPO) | in vitro E.coli expression system |
| CSB-MP764926PI | Recombinant Pig Translocator protein (TSPO)-VLPs | Mammalian cell |
TSPO Proteins for Ovis aries (Sheep)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP872394SH1 CSB-EP872394SH1 CSB-BP872394SH1 CSB-MP872394SH1 CSB-EP872394SH1-B |
Recombinant Sheep Translocator protein (TSPO), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF872394SH | Recombinant Sheep Translocator protein (TSPO) | in vitro E.coli expression system |
