HSPA8
What is HSPA8?
Heat shock cognate protein 70 (HSC70), also known as HSP73, is a heat shock homologous protein with molecular weight of 73kDa. It is a constitutively expressed molecular chaperone encoded by HSPA8 gene.
Unlike HSP70, HSC70 can be expressed in the absence of any stress, mainly located in the cytoplasm and nucleolus.
As a chaperone, HSC70 can provide a variety of housekeeping functions. It can regulate the folding, transport, migration and collection of proteins, especially to help the refolding of misfolded proteins, so that the function of proteins can return to normal. In addition, it participates in the inflammatory signaling pathway through extracellular interaction with TLR2/TLR4.
The Structure of HSPA8
The HSC70 gene contains 8 introns, and its cDNA sequence has been found in human, rat, mouse, Chinese hamster and cattle cells. It is speculated that the protein encoded by the gene contains about 646 amino acids and the predicted molecular weight is about 73 kDa.
The basic structure of human HSC70 consists of three parts:
44kDa N-terminal adenosine triphosphatase (ATPase) domain (AA 1 - 384), also known as ATP binding domain.
18kDa polypeptide (substrate) binding domain (AA 385 - 543).
10kDa carboxyl terminal domain (AA 544 - 646).
Carboxyl terminal amino acid sequence Glu-Glu-Val-Asp (EEVD motif) is highly conserved in HSC70 and HSP70 of all eukaryotes and plays an important role in association with some molecular chaperones. This conserved motif is beneficial to the binding of HSC70 and HSP70 to other chaperone molecules. The terminal amino acid sequence is specific in different organelles, such as EEVD, in the cytoplasm, HDEL, in the endoplasmic reticulum, PEAEYEEAKK, in the mitochondria and PECDVLDADFTDFTDSK in the plastids.
The Expression of HSPA8
HSC70 exists in all prokaryotes and eukaryotes, and shows basic expression in normal cells, thus maintaining the basic morphology and function of cells. Under the stimulation of environmental stress factors (such as high temperature, heavy metals and microbial infection, etc.), its expression increased significantly.
HSC70 is highly expressed in the nervous system, and its expression changes in time and space with the development of the nervous system, suggesting that it is involved in neural development.
Compared with normal cell lines, the expression of HSC70 is higher in some cancer cell lines. Compared with normal mucosal epithelial cells, HSC70 was highly expressed in cervical squamous cell carcinoma and vaginal squamous cell carcinoma. The expression of HSC70 in colon cancer tissues was significantly higher than that in adjacent normal tissues. The expression of HSC70 in esophageal squamous cell carcinoma was also significantly increased.
The Function of HSPA8
HSC70 is fundamentally expressed in normal cells and plays a role in the normal metabolism and physiological regulation of cells.
HSC70 is an important cytoplasmic molecular chaperone protective protein, which is widely involved in a variety of functions to protect the body and cells, enhance the body's stress tolerance and improve cell survival.
As a molecular chaperone, it is involved in a variety of cellular processes, including the folding of new peptides, protein transmembrane transport, proteolysis activation of misfolded proteins, and the formation and dissociation of protein complexes.
In addition to chaperone function, secreted HSC70 also participates in inflammatory signaling pathway through extracellular interaction with TLR2/TLR4. During nutritional stress, it also plays a central role in regulating intracellular antigen transport to control MHC class II presentation. In addition, HSC70 may act as a molecular switch to regulate endocytosis and autophagy.
HSC70 is highly expressed in mammalian nervous system and neuronal cell bodies, and temporal and spatial regulation occurs with the development of nervous system, indicating that it is involved in neural development.
It has been found that HSC70 can mediate the infection and release of hepatitis C virus (HCV) particles, and it is also an important helper protein for HBV replication. The role of heat shock proteins in virus replication provides a new idea for the research of antiviral drugs.
HSC70 plays an important role in the initiation of labor.
The important function of HSC70 also makes it closely related to a variety of diseases.
HSC70 plays an important role in regulating the innate immune response of ischemia-reperfusion myocardium and protecting cardiac function. In addition, HSC70 plays an important role in neurological diseases, liver diseases, breast cancer and other cancers.
The Latest Research Progress
Research shows miR-26b-5p imparts metastatic properties and helps in maintenance of Ep CSCs via HSC70. Thus, miR-26b-5p and HSC70 could serve as molecular targets for selectively eliminating the Ep CSC population in human HCCs.
Hot Products of HSPA8
HSPA8 Monoclonal Antibody (ELISA, WB, IHC, FC, IP) (CSB-MA010829A0m)
HSPA8 Antibodies
HSPA8 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-PA010829GA01HU | HSPA8 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA162061 | HSPA8 Antibody | Human | ELISA,WB |
| CSB-PA002972 | HSPA8 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA002973 | HSPA8 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA080152 | HSPA8 Antibody | Human,Mouse,Rat | WB, IHC |
| CSB-PA575152 | HSPA8 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA03249A0Rb | HSPA8 Antibody | Human, Mouse, Rat | ELISA, WB, IHC |
| CSB-PA03249B0Rb | HSPA8 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA03249C0Rb | HSPA8 Antibody, FITC conjugated | Human | |
| CSB-PA03249D0Rb | HSPA8 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA010829A0HU | HSPA8 Recombinant Monoclonal Antibody | Human | ELISA, WB |
| CSB-MA010829A0m | HSPA8 Monoclonal Antibody | Human, Rat, Mouse | ELISA, WB, IHC, FC, IP |
| CSB-MA010829A1m | HSPA8 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
HSPA8 for H,R,M
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-MA080276 | HSPA8 Monoclonal Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-MA080291 | HSPA8 Monoclonal Antibody | Human,Mouse,Rat | ELISA,WB |
HSPA8 Proteins
HSPA8 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829HU | Recombinant Human Heat shock cognate 71 kDa protein (HSPA8) | Yeast |
| CSB-EP010829HU CSB-BP010829HU CSB-MP010829HU CSB-EP010829HU-B |
Recombinant Human Heat shock cognate 71 kDa protein (HSPA8) | E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829BO CSB-EP010829BO CSB-BP010829BO CSB-MP010829BO CSB-EP010829BO-B |
Recombinant Bovine Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829RA CSB-EP010829RA CSB-BP010829RA CSB-MP010829RA CSB-EP010829RA-B |
Recombinant Rat Heat shock cognate 71 kDa protein (Hspa8) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-BP010829MO CSB-MP010829MO CSB-EP010829MO-B |
Recombinant Mouse Heat shock cognate 71 kDa protein (Hspa8) | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Equus caballus (Horse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829HO CSB-EP010829HO CSB-BP010829HO CSB-MP010829HO CSB-EP010829HO-B |
Recombinant Horse Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Gallus gallus (Chicken)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829CH CSB-EP010829CH CSB-BP010829CH CSB-MP010829CH CSB-EP010829CH-B |
Recombinant Chicken Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP010829DXU CSB-EP010829DXU CSB-BP010829DXU CSB-MP010829DXU CSB-EP010829DXU-B |
Recombinant Cricetulus griseus Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Pongo abelii (Sumatran orangutan)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP711856PYX CSB-EP711856PYX CSB-BP711856PYX CSB-MP711856PYX CSB-EP711856PYX-B |
Recombinant Pongo abelii Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Saguinus oedipus (Cotton-top tamarin)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP761741SWB CSB-EP761741SWB CSB-BP761741SWB CSB-MP761741SWB CSB-EP761741SWB-B |
Recombinant Saguinus oedipus Heat shock cognate 71 kDa protein (HSPA8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP852659DIL CSB-EP852659DIL CSB-BP852659DIL CSB-MP852659DIL CSB-EP852659DIL-B |
Recombinant Danio rerio Heat shock cognate 71 kDa protein (hspa8), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
HSPA8 ELISA Kit
HSPA8 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 樣本類(lèi)型 | 靈敏度 |
|---|---|---|---|
| CSB-EL010829HU | Human Heat shock cognate 71 kDa protein(HSPA8) ELISA kit | serum, plasma, tissue homogenates | 7.8 pg/mL |
