ERBB2
ERBB2，即人表皮生長因子受體2，別名NEU、HER-2、CD340、HER2，由人類ERBB2基因編碼，該基因位于7號染色體上。ERBB2蛋白是一種跨膜酪氨酸激酶受體，屬于HER家族成員之一，由胞外配體結合域、跨膜結構域和胞內結構域三部分組成。其作用機制涉及與其他HER家族成員結合形成異二聚體，增強激酶的激活作用并介導下游信號通路的活性，如MAPK和PI3K/AKT信號通路。
ERBB2的異常激活，包括基因擴增、過表達和突變，與多種癌癥的發生發展有關，如乳腺癌、非小細胞肺癌、胃癌和子宮內膜腫瘤等。ERBB2基因擴增與乳腺癌的預后不良相關，已成為近30%乳腺癌患者生物治療的重要靶點。
熱銷產品
Recombinant Human Epidermal growth factor receptor (EGFR), partial (Active) (CSB-MP007479HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP007479HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) EGFR.
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Anti-EGFR recombinant antibody, the EC50 of human EGFR protein is 2.867-3.571 ng/ml.
Human EGF protein captured on COOH chip can bind Human EGFR protein, his and Myc tag (CSB-MP007479HU) with an affinity constant of 11.9nM as detected by LSPR Assay.
Activity
Measured by its binding ability in a functional ELISA. Immobilized EGFR at 1 μg/ml can bind Cetuximab, the EC50 of human EGFR protein is 0.6919-1.047 ng/ml.
The purity of EGFR was greater than 95% as determined by SEC-HPLC
EGFR Recombinant Monoclonal Antibody (CSB-RA159341MA1HU)
驗證數據
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA159341MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA159341MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
ERBB2 Antibodies
ERBB2 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA007763GA01HU | ERBB2 Antibody | Human | ELISA,WB,IHC |
| CSB-PA09759C0Rb | ERBB2 Antibody, FITC conjugated | Human | |
| CSB-PA09759D0Rb | ERBB2 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA007763KA01HU | ERBB2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA09759B0Rb | ERBB2 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA09759A0Rb | ERBB2 Antibody | Human | ELISA, IHC |
| CSB-PA846722 | Phospho-ERBB2 (Tyr877) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA281422 | Phospho-ERBB2 (Tyr1221/Tyr1222) Antibody | Human | ELISA,WB,IF |
| CSB-PA997735 | Phospho-ERBB2 (Tyr1248) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA200841 | ERBB2 (Ab-877) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA899547 | ERBB2 (Ab-1221/1222) Antibody | Human | ELISA,WB,IHC,IF |
| CSB-PA115264 | ERBB2 (Ab-1248) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
| CSB-PA761682 | Phospho-ERBB2 (Tyr1112) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA000577 | Phospho-ERBB2 (Y1221/Y1222) Antibody | Human,Mouse,Rat | WB, IHC, IP, ELISA |
| CSB-PA000578 | Phospho-ERBB2 (Y877) Antibody | Human,Mouse,Rat | WB, IHC, IP, ELISA |
| CSB-PA000733 | Phospho-ERBB2 (Y1221/Y1222) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
| CSB-PA003405 | ERBB2 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA003407 | ERBB2 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA008077 | Phospho-ERBB2 (Y1248) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA008078 | Phospho-ERBB2 (Y1112) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA008079 | Phospho-ERBB2 (T686) Antibody | Human,Mouse,Rat | IF, ELISA |
| CSB-PA008081 | ERBB2 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA008088 | ERBB2 Antibody | Human,Mouse,Rat | IF, ELISA |
| CSB-PA008089 | ERBB2 Antibody | Human,Mouse,Rat | IF, ELISA |
| CSB-PA008092 | ERBB2 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA972166 | ERBB2 Antibody | Human | ELISA,WB |
| CSB-RA260392A0HU | ERBB2 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF |
| CSB-RA007763MA1HU | ERBB2 (Trastuzumab/herceptin) Recombinant Monoclonal Antibody | Human | ELISA |
| CSB-RA588766A0HU | Phospho-ERBB2 (Y1139) Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF |
| CSB-RA007763MA2HU | ERBB2 Recombinant Monoclonal Antibody | Human | ELISA, IHC |
ERBB2 for H, M, R
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-MA000200 | ERBB2 Monoclonal Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
ERBB2 for H
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-MA000237 | ERBB2 Monoclonal Antibody | Human | ELISA,WB |
ERBB2 Proteins
ERBB2 Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP007763MO CSB-EP007763MO CSB-BP007763MO CSB-MP007763MO CSB-EP007763MO-B |
Recombinant Mouse Receptor tyrosine-protein kinase erbB-2 (Erbb2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP007763RA CSB-EP007763RA CSB-BP007763RA CSB-MP007763RA CSB-EP007763RA-B |
Recombinant Rat Receptor tyrosine-protein kinase erbB-2 (Erbb2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP007763HU CSB-BP007763HU CSB-EP007763HU-B |
Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | Yeast Baculovirus In Vivo Biotinylation in E.coli |
| CSB-EP007763HU1 | Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | E.coli |
| CSB-EP007763HU | Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | E.coli |
| CSB-EP007763HU3 | Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | E.coli |
| CSB-MP007763HU | Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial (Active) | Mammalian cell |
| CSB-MP007763HU(M) | Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2)(S310Y), partial | Mammalian cell |
ERBB2 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP007763DO CSB-EP007763DO CSB-BP007763DO CSB-MP007763DO CSB-EP007763DO-B |
Recombinant Dog Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Mesocricetus auratus (Golden hamster)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP736718MRG CSB-EP736718MRG CSB-BP736718MRG CSB-MP736718MRG CSB-EP736718MRG-B |
Recombinant Mesocricetus auratus Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 ELISA Kit
ERBB2 ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E11161h | Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit | serum, plasma, tissue homogenates, cell lysates | 0.04 ng/mL |
| CSB-E11161h-IS | Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit | serum, plasma, cell culture supernates, cell lysates, tissue homogenates | 3.9 pg/mL |
