DHFR
DHFR，即二氫葉酸還原酶，別名dihydrofolatereductase。這是一種在人類細(xì)胞中廣泛表達(dá)的酶，對(duì)于嘌呤和嘧啶的生物合成至關(guān)重要。DHFR的作用機(jī)制是利用NADPH作為電子供體，催化二氫葉酸還原為四氫葉酸，后者是DNA合成和修復(fù)過程中必需的輔酶。DHFR的活性對(duì)于細(xì)胞的生長和分裂非常重要，因?yàn)樗鼌⑴c了一碳單位的轉(zhuǎn)移反應(yīng)，這對(duì)于氨基酸的合成和核酸的合成都是必不可少的。DHFR也是一些抗癌藥物如氨甲喋呤（MTX）的作用靶點(diǎn)，這些藥物通過抑制DHFR的活性來阻止癌細(xì)胞的增殖。DHFR的異常表達(dá)或功能失調(diào)與多種疾病相關(guān)，包括某些類型的貧血和癌癥。
熱銷產(chǎn)品
Recombinant Human Dihydrofolate reductase (DHFR) (CSB-EP006847HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
DHFR Recombinant Monoclonal Antibody (CSB-RA264582A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue
All lanes: DHFR antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22, 16 kDa
Observed band size: 22 kDa
IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating DHFR in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA264582A0HU in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA264582A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)
DHFR Antibodies
DHFR for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA006847GA01HU | DHFR Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA006847HA01HU | DHFR Antibody | Human | ELISA, IHC, IF |
| CSB-RA264582A0HU | DHFR Recombinant Monoclonal Antibody | Human, Mouse, Rat | ELISA, WB, IHC, IP |
DHFR Proteins
DHFR Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP006847HU CSB-BP006847HU CSB-MP006847HU CSB-EP006847HU-B |
Recombinant Human Dihydrofolate reductase (DHFR) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP006847HU1 | Recombinant Human Dihydrofolate reductase (DHFR), partial | E.coli |
| CSB-EP006847HU | Recombinant Human Dihydrofolate reductase (DHFR) | E.coli |
