CD300A
CD300A，全稱(chēng)為CMRF35-like molecule 8，是一種廣泛表達(dá)的免疫調(diào)節(jié)蛋白，在自然殺傷細(xì)胞、T細(xì)胞亞群、B細(xì)胞、樹(shù)突細(xì)胞、肥大細(xì)胞等多種免疫細(xì)胞表面均有表達(dá)。該蛋白具有免疫受體酪氨酸基抑制基序，通過(guò)與磷脂酰絲氨酸等分子的相互作用，負(fù)性調(diào)節(jié)NK細(xì)胞的細(xì)胞毒性活性，并抑制肥大細(xì)胞的脫顆粒反應(yīng)。CD300A蛋白還參與調(diào)控Toll樣受體信號(hào)通路，通過(guò)激活PTPN6蛋白，負(fù)向調(diào)控MYD88介導(dǎo)的TLR信號(hào)傳導(dǎo)，從而影響免疫細(xì)胞的活化和細(xì)胞因子的產(chǎn)生。研究表明，CD300A蛋白的表達(dá)異常與血液系統(tǒng)惡性腫瘤的發(fā)生、發(fā)展密切相關(guān)，其高表達(dá)與患者生存期縮短和腫瘤微環(huán)境中NK細(xì)胞的耗竭表型相關(guān)。針對(duì)CD300A蛋白的藥物研發(fā)正在積極進(jìn)行中，例如通過(guò)阻斷CD300A與配體的相互作用，增強(qiáng)NK細(xì)胞對(duì)癌細(xì)胞的殺傷能力，為血液惡性腫瘤和其他類(lèi)型癌癥的治療提供了新的思路。
熱銷(xiāo)產(chǎn)品
Recombinant Human CMRF35-like molecule 8 (CD300A), partial (CSB-EP887023HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CD300A Antibody (CSB-PA887023ESR1HU)
驗(yàn)證數(shù)據(jù)
Western Blot Positive WB detected in: THP-1 whole cell lysate(30μg), HL-60 whole cell lysate(30μg), JK whole cell lysate(30μg), K562 whole cell lysate(30μg), Raji whole cell lysate(30μg), Mouse Spleen tissue lysate(30μg),Rat Spleen tissue lysate(30μg) All lanes: CD300A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 34,21,12,17 kDa Observed band size: 60 kDa Exposure time: 120s
IHC image of CSB-PA887023ESR1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Immunofluorescence staining of HepG2 cell with CSB-PA887023ESR1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
CD300A Antibodies
CD300A for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 種屬反應(yīng)性 | 應(yīng)用類(lèi)型 |
|---|---|---|---|
| CSB-PA887023ESR1HU | CD300A Antibody | Human, Mouse,Rat | ELISA, WB, IHC, IF |
| CSB-PA887023ESR2HU | CD300A Antibody | Human | ELISA, IHC |
CD300A Proteins
CD300A Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
|---|---|---|
| CSB-YP887023HU CSB-BP887023HU CSB-MP887023HU CSB-EP887023HU-B |
Recombinant Human CMRF35-like molecule 8 (CD300A), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP887023HU | Recombinant Human CMRF35-like molecule 8 (CD300A), partial | E.coli |
