CALR

CALR，全稱為鈣網(wǎng)蛋白（Calreticulin），是一種高度保守的內(nèi)質(zhì)網(wǎng)蛋白，主要參與蛋白質(zhì)的折疊、組裝和質(zhì)量控制。它通過與單葡萄糖基化糖蛋白相互作用，促進內(nèi)質(zhì)網(wǎng)中蛋白質(zhì)的正確折疊，并介導(dǎo)NR3C1 DNA結(jié)合結(jié)構(gòu)域的核輸出。CALR蛋白還參與細胞應(yīng)激反應(yīng)和免疫調(diào)節(jié)，如與自身免疫性疾病的發(fā)生發(fā)展密切相關(guān)。在藥物研發(fā)方面，CALR蛋白已成為治療自身免疫性疾病和癌癥的重要靶點。例如，Cartesian Therapeutics公司開發(fā)的mRNA CAR-T細胞療法Descartes-08，通過靶向B細胞成熟抗原(BCMA)，清除分泌自身抗體的漿細胞，在治療慢性自身免疫性疾病全身型重癥肌無力(MG)患者中取得了顯著療效。此外，Calreticulin/CALR蛋白也被用于開發(fā)針對急性髓系白血病（AML）的CAR-T療法，如羅斯威爾帕克癌癥研究所開發(fā)的抗CD83 CAR-T療法，已在1期臨床試驗階段，為AML患者提供了新的治療選擇。

熱銷產(chǎn)品

Recombinant Human Calreticulin (CALR) (CSB-MP004458HU)

驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CALR Recombinant Monoclonal Antibody (CSB-RA004458MA1HU)

驗證數(shù)據(jù)

Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: CALR antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

IHC image of CSB-RA004458MA1HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 cell with CSB-RA004458MA1HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay Peak curve showing JK cells stained with CSB-RA004458MA1HU (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,018 events was performed.

Human Calreticulin,CRT ELISA Kit (CSB-E09787h)

驗證數(shù)據(jù)

貨號：CSB-E09787h

規(guī)格：96T/48T

靈敏度：0.039 ng/mL

檢測范圍：0.156 ng/mL-10 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

CALR Antibody

CALR Protein

CALR ELISA Kit

CALR Antibodies

CALR for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA07864A0Rb	CALR Antibody	Human	ELISA, WB, IHC
CSB-PA07864D0Rb	CALR Antibody, Biotin conjugated	Human	ELISA
CSB-PA07864B0Rb	CALR Antibody, HRP conjugated	Human	ELISA
CSB-PA07864C0Rb	CALR Antibody, FITC conjugated	Human
CSB-PA162228	CALR Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA001196	CALR Antibody	Human,Mouse,Rat,Monkey	WB, IHC, IF, ELISA
CSB-PA07867A0Rb	CALR Antibody	Human, Mouse, Rat	ELISA, WB, IHC, IF
CSB-PA07867B0Rb	CALR Antibody, HRP conjugated	Human	ELISA
CSB-PA07867C0Rb	CALR Antibody, FITC conjugated	Human
CSB-PA07867D0Rb	CALR Antibody, Biotin conjugated	Human	ELISA
CSB-PA314121	CALR Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA963019	CALR Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA004458YA01HU	CALR Antibody	Human, Mouse	ELISA, WB, IHC, IF
CSB-PA004458YB01HU	CALR Antibody, HRP conjugated	Human	ELISA
CSB-PA004458YC01HU	CALR Antibody, FITC conjugated	Human
CSB-PA004458OA01HU	CALR Antibody	Human	ELISA, WB
CSB-PA004458OB01HU	CALR Antibody, HRP conjugated	Human	ELISA
CSB-PA004458OC01HU	CALR Antibody, FITC conjugated	Human
CSB-PA004458OD01HU	CALR Antibody, Biotin conjugated	Human	ELISA
CSB-RA004458MA1HU	CALR Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, IHC, IF, FC

CALR for Pig

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA004458YA01PI	CALR Antibody	Pig	ELISA
CSB-PA004458YD01PI	CALR Antibody, Biotin conjugated	Pig	ELISA

CALR Proteins

CALR Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458MO	Recombinant Mouse Calreticulin (Calr)	Yeast
CSB-EP004458MO	Recombinant Mouse Calreticulin (Calr)	E.coli
CSB-BP004458MO CSB-MP004458MO CSB-EP004458MO-B	Recombinant Mouse Calreticulin (Calr)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP004458MOc7	Recombinant Mouse Calreticulin (Calr)	Yeast

CALR Proteins for Oryctolagus cuniculus (Rabbit)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458RB CSB-EP004458RB CSB-BP004458RB CSB-MP004458RB CSB-EP004458RB-B	Recombinant Rabbit Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-BP004458HU CSB-EP004458HU-B	Recombinant Human Calreticulin (CALR)	Baculovirus In Vivo Biotinylation in E.coli
CSB-EP004458HU	Recombinant Human Calreticulin (CALR)	E.coli
CSB-EP004458HUe0	Recombinant Human Calreticulin (CALR)	E.coli
CSB-EP004458HU1	Recombinant Human Calreticulin (CALR), partial	E.coli
CSB-MP004458HU	Recombinant Human Calreticulin (CALR)	Mammalian cell
CSB-YP004458HU	Recombinant Human Calreticulin (CALR)	Yeast
CSB-MP004458HUd6	Recombinant Human Calreticulin (CALR)	Mammalian cell

CALR Proteins for Sus scrofa (Pig)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458PI	Recombinant Pig Calreticulin (CALR)	Yeast
CSB-EP004458PI CSB-BP004458PI CSB-MP004458PI CSB-EP004458PI-B	Recombinant Pig Calreticulin (CALR)	E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458DO CSB-EP004458DO CSB-BP004458DO CSB-MP004458DO CSB-EP004458DO-B	Recombinant Dog Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Bos taurus (Bovine)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458BO CSB-EP004458BO CSB-BP004458BO CSB-MP004458BO CSB-EP004458BO-B	Recombinant Bovine Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458RA CSB-EP004458RA CSB-BP004458RA CSB-MP004458RA CSB-EP004458RA-B	Recombinant Rat Calreticulin (Calr)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458MOV CSB-EP004458MOV CSB-BP004458MOV CSB-MP004458MOV CSB-EP004458MOV-B	Recombinant Macaca fascicularis Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-EP004458DXU CSB-BP004458DXU CSB-MP004458DXU CSB-EP004458DXU-B	Recombinant Cricetulus griseus Calreticulin (CALR)	E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP004458DXU	Recombinant Cricetulus griseus Calreticulin (CALR)	Yeast

CALR Proteins for Macaca fuscata fuscata (Japanese macaque)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458MMX CSB-EP004458MMX CSB-BP004458MMX CSB-MP004458MMX CSB-EP004458MMX-B	Recombinant Macaca fuscata fuscata Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CALR Proteins for Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP004458DSU CSB-EP004458DSU CSB-BP004458DSU CSB-MP004458DSU CSB-EP004458DSU-B	Recombinant Chlorocebus aethiops Calreticulin (CALR)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli