ADGRE5
ADGRE5（Adhesion G Protein-Coupled Receptor E5）又名CD97，屬于粘附類G蛋白偶聯受體家族，其蛋白結構包含胞外EGF樣結構域和跨膜七次螺旋結構，能夠通過結合補體調節蛋白CD55等配體介導細胞間黏附。該蛋白在單核細胞、巨噬細胞等免疫細胞中高表達，參與調控炎癥反應、細胞遷移及免疫微環境重塑。ADGRE5通過激活Wnt/β-catenin、PI3K/AKT等通路，與結直腸癌、胰腺癌等實體瘤的侵襲轉移密切相關，其過表達可促進腫瘤血管生成和免疫逃逸。在動脈粥樣硬化領域，ADGRE5通過與血管平滑肌細胞相互作用加速斑塊進展。近年來針對該靶點的藥物開發聚焦于單克隆抗體和拮抗肽，已有研究團隊開發出阻斷CD97-CD55互作的抗體藥物，在動物模型中顯示出抑制腫瘤轉移的效果。
熱銷產品
CD97 Recombinant Monoclonal Antibody (CSB-RA004972A0HU)
驗證數據
IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
ADGRE5 Antibodies
ADGRE5 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA004972GA01HU | CD97 Antibody | Human | ELISA,WB,IHC |
| CSB-PA080060 | Cleaved-CD97 (L530) Antibody | Human | WB, ELISA |
| CSB-PA080061 | Cleaved-CD97 (S531) Antibody | Human | WB, ELISA |
| CSB-PA558771 | CD97 Antibody | Human | ELISA,WB,IHC |
| CSB-PA563396 | CD97 Antibody | Human | ELISA,IHC |
| CSB-PA004972ESR2HU | CD97 Antibody | Human | ELISA, WB, IHC |
| CSB-RA004972A0HU | CD97 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF |
ADGRE5 Proteins
ADGRE5 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP004972HU1 CSB-EP004972HU1 CSB-BP004972HU1 CSB-MP004972HU1 CSB-EP004972HU1-B |
Recombinant Human Adhesion G protein-coupled receptor E5 (ADGRE5), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF004972HU | Recombinant Human Adhesion G protein-coupled receptor E5 (ADGRE5) | in vitro E.coli expression system |
ADGRE5 Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP823366BO1 CSB-EP823366BO1 CSB-BP823366BO1 CSB-MP823366BO1 CSB-EP823366BO1-B |
Recombinant Bovine CD97 antigen (CD97), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF823366BO | Recombinant Bovine CD97 antigen (CD97) | in vitro E.coli expression system |
