ADAM10
ADAM10全稱為解整合素-金屬蛋白酶10，屬于跨膜蛋白家族成員，在細(xì)胞膜表面發(fā)揮關(guān)鍵切割酶功能。ADAM10通過(guò)裂解Notch、E-鈣黏蛋白等重要膜蛋白，調(diào)控細(xì)胞分化與黏附過(guò)程，其異常活化會(huì)破壞細(xì)胞間通訊穩(wěn)態(tài)。在阿爾茨海默病中，ADAM10過(guò)度切割淀粉樣前體蛋白導(dǎo)致β淀粉樣斑塊沉積，已成為該病治療的重要靶點(diǎn)。腫瘤領(lǐng)域研究發(fā)現(xiàn)，該蛋白通過(guò)釋放表皮生長(zhǎng)因子受體配體促進(jìn)癌細(xì)胞侵襲轉(zhuǎn)移，在乳腺癌、結(jié)腸癌等惡性腫瘤中呈現(xiàn)高表達(dá)特征。針對(duì)ADAM10的小分子抑制劑（如GI254023X）和單克隆抗體（如LY503307）已進(jìn)入臨床前研究階段，其中部分藥物在動(dòng)物模型中顯示出抑制腫瘤生長(zhǎng)和延緩認(rèn)知衰退的效果，但需解決選擇性差和血腦屏障穿透難題。ADAM10在免疫調(diào)節(jié)和神經(jīng)發(fā)育中的雙重作用使其藥物開(kāi)發(fā)面臨復(fù)雜挑戰(zhàn)。
熱銷產(chǎn)品
ADAM10 Recombinant Monoclonal Antibody (CSB-RA001273MA1HU)
驗(yàn)證數(shù)據(jù)
Overlay Peak curve showing Hela cells stained with CSB-RA001273MA1HU (red line) at 1:400. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
ADAM10 Antibody (CSB-PA001273LA01HU)
驗(yàn)證數(shù)據(jù)
Immunofluorescence staining of Hela cells with CSB-PA001273LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001273LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thymus tissue using CSB-PA001273LA01HU at dilution of 1:100
ADAM10 Antibodies
ADAM10 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA000013 | ADAM10 Antibody | Human | WB, ELISA |
| CSB-PA069457 | ADAM10 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-PA909378 | ADAM10 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA001273LA01HU | ADAM10 Antibody | Human | ELISA, IHC, IF |
| CSB-PA001273LD01HU | ADAM10 Antibody, Biotin conjugated | Human | ELISA |
| CSB-PA001273LB01HU | ADAM10 Antibody, HRP conjugated | Human | ELISA |
| CSB-RA001273MA1HU | ADAM10 Recombinant Monoclonal Antibody | Human | ELISA, FC |
ADAM10 Proteins
ADAM10 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP001273HU1 CSB-EP001273HU1 CSB-BP001273HU1 CSB-MP001273HU1 CSB-EP001273HU1-B |
Recombinant Human Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF001273HU | Recombinant Human Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) | in vitro E.coli expression system |
ADAM10 ELISA Kit
ADAM10 ELISA Kit for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E11172h | Human A Disintegrin And Metalloprotease 10,ADAM10 ELISA Kit | serum, plasma, tissue homogenates | 1.95 pg/mL |
