Mouse factor B,BF ELISA Kit

Mouse factor B,BF ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

小鼠B因子(BF)酶聯免疫試劑盒
貨號：

CSB-E04695m
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

小鼠B因子(BF)酶聯免疫試劑盒（CSB-E04695m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的BF含量。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，科研人員可將其應用于小鼠感染模型、自身免疫疾病造模、藥物干預實驗等場景中，定量分析血清或血漿BF水平變化，為探索補體系統在先天免疫中的作用、炎癥反應調控機制或免疫調節劑開發提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cfb ELISA kit; Bf ELISA kit; H2-BfComplement factor B ELISA kit; EC 3.4.21.47 ELISA kit; C3/C5 convertase) [Cleaved into: Complement factor B Ba fragment; Complement factor B Bb fragment] ELISA kit
縮寫：

BF
Uniprot No.：

P04186
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.078 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-104
1:200	Average %	95
	Range %	91-99
1:400	Average %	97
	Range %	91-105
1:800	Average %	102
	Range %	96-108

回收率：

The recovery of mouse BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-94
EDTA plasma (n=4)	90	85-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
20	2.712	2.811	2.762	2.608
10	2.112	2.212	2.162	2.008
5	1.428	1.447	1.438	1.284
2.5	0.857	0.866	0.862	0.708
1.25	0.524	0.512	0.518	0.364
0.625	0.350	0.341	0.346	0.192
0.312	0.268	0.263	0.266	0.112
0	0.154	0.153	0.154

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Elevated activity of plasma dipeptidyl peptidase 4 upon stress can be targeted to reverse tumor immunosuppression P Li, M Liang, J Zhu, J Chen, L Xia, Z Jin,Pharmacological Research,2025

功能：

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
數據庫鏈接：

STRING: 10090.ENSMUSP00000025229

UniGene: Mm.653

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.