Recombinant Saccharomyces cerevisiae Palmitoyltransferase PFA3 (PFA3)

in vitro E.coli expression system

中文名稱：

釀酒酵母PFA3重組蛋白
貨號(hào)：

CSB-CF337234SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

PFA3
Uniprot No.：

P42836
別名：

PFA3; YNL326C; N0325; Palmitoyltransferase PFA3; Protein fatty acyltransferase 3
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-336
氨基酸序列

MNDRLSLTSLFPRCLTTCLYIWTAYITLTRIHQIPRWFLALTIVPTLAVALYTYYKVIAR GPGSPLDFPDLLVHDLKAAENGLELPPEYMSKRCLTLKHDGRFRVCQVCHVWKPDRCHHC SSCDVCILKMDHHCPWFAECTGFRNQKFFIQFLMYTTLYAFLVLIYTCYELGTWFNSGSF NRELIDFHLLGVALLAVAVFISVLAFTCFSIYQVCKNQTTIEVHGMRRYRRDLEILNDSY GTNEHLENIFDLGSSMANWQDIMGTSWLEWILPIETFKYKKSKHTKDEKGLYFNVRPQVQ DRLLSSRCLEDQLLRRVTPRPSLEADRASVEIIDAN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Palmitoyltransferase specific for VAC8. Palmitoylates VAC8 at one or more of its N-terminal cysteine residues, which is required for its proper membrane localization.
基因功能參考文獻(xiàn)：
1. This study focusses on the function of PFA3 as the third member of the DHHC-CRD family found to have protein acyltransferase activity. PMID: 16186255
2. The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8. PMID: 16301533
3. Data show that for Pfa3 and its substrate Vac8, there are two consecutive steps in the acylation reaction: initial binding independent of cysteine in the DHHC box, but requiring myristoylation of Vac8, and a DHHC-motif dependent acylation. PMID: 19453970
亞細(xì)胞定位：

Vacuole membrane; Multi-pass membrane protein.
蛋白家族：

DHHC palmitoyltransferase family, PFA3 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:YNL326C

STRING: 4932.YNL326C