Recombinant Rhizobium meliloti C4-dicarboxylate transport sensor protein dctB (dctB)

in vitro E.coli expression system

中文名稱：

Recombinant Rhizobium meliloti C4-dicarboxylate transport sensor protein dctB(dctB)
貨號：

CSB-CF318394RKU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

dctB
Uniprot No.：

P13633
別名：

dctB; RB1524; SMb20612; C4-dicarboxylate transport sensor protein DctB
種屬：

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
蛋白長度：

full length protein
表達區域：

1-621
氨基酸序列

MHHVRMVKLPAEASDPHALRSRARRSWLVFAAVALVLLAAGLLLARDYGRSQALAGLAGQ SRIDASLKASLLRAVVERQRALPLVLADDAAIRGALLSPDRPSLDRINRKLEALATSAEA AVIYLIDRSGVAVAASNWQEPTSFVGNDYAFRDYFRLAVRDGMAEHFAMGTVSNRPGLYI SRRVDGPGGPLGVIVAKLEFDGVEADWQASGKPAYVTDRRGIVLITSLPSWRFMTTKPIA EDRLAPIRESLQFGDAPLLPLPFRKIEARPDGSSTLDALLPGDSTAAFLRVETMVPSTNW RLEQLSPLKAPLAAGAREAQLLTLAALVPLLALAALLLRRRQVVAMRSAEERLARNALEA SVEERTRDLRMARDRLETEIADHRQTTEKLQAVQQDLVQANRLAILGQVAAGVAHEINQP VATIRAYADNARTFLHRGQTVTAAENMESIAELTERVGAITDELRRFARKGHFAAGPTAM KEVVEGALMLLRSRFAGRMDAIRLDLPPDGLQALGNRIRLEQVLINLLQNALEAIGDSED GAIQVRCEEAAGGIALTVADNGPGIAADVREELFTPFNTSKEDGLGLGLAISKEIVSDYG GTIEVESGPSGTTFAVNLKKA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Member of the two-component regulatory system DctB/DctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates DctD in response to environmental signals.
基因功能參考文獻：
1. These results suggest that DctBp's signal perception and transduction occur through a 'ligand-induced dimeric switch', in which the changes in the dimeric conformations upon ligand binding are responsible for the signal transduction in DctB. PMID: 20149110
2. The crystals diffracted to 2.3 A resolution and belonged to P42 space group with unit cell dimensions of a = b = 71.77 A, c = 227.14 A. The asymmetric unit contains four molecules with a corresponding VM of 2.4 A3 Da(-1) and a solvent content of 49.1%. PMID: 16332458
3. structures of the different ligand binding states revealed conformational changes initiated upon ligand binding and propagated to the N-terminal domain responsible for dimerization, providing insights into the mechanism of signal transduction PMID: 18725229
亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
數據庫鏈接：

KEGG: sme:SM_b20612