Recombinant Rat Muscarinic acetylcholine receptor M3 (Chrm3)

in vitro E.coli expression system

中文名稱：

Recombinant Rat Muscarinic acetylcholine receptor M3(Chrm3)
貨號(hào)：

CSB-CF005383RA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Chrm3
Uniprot No.：

P08483
別名：

Chrm3; Chrm-3; Muscarinic acetylcholine receptor M3
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Full length protein
表達(dá)區(qū)域：

1-589
氨基酸序列

MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLG GHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVIS MNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRT TKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFY MPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVK RSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSET RAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQ KDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRM SLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNP VCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
基因功能參考文獻(xiàn)：
1. Data suggest that, in atrial myocardium (or isolated cardiomyocytes), activation of muscarinic receptors M3 (Chrm3) leads to shortening of action potentials via suppression of L-type calcium channels; the major potassium channels are not affected, but inwardly rectifying potassium channels are suppressed. PMID: 27858307
2. M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product. PMID: 26626425
3. Our results suggest that the mechanism for the deficits in learning and memory of rats with chronic fluorosis may be associated with the decreased expressions of M1 and M3 in mAChRs PMID: 25417050
4. The expression of beta3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat. PMID: 25920933
5. The ventricular myocardium of rat pups differs from that of mature rats by a far greater sensitivity to selective stimulation of M3 receptors, suggesting an important functional role of M3 receptors in the newborn rats, which declines with age. PMID: 26033578
6. Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats. PMID: 25474381
7. Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis. PMID: 25681120
8. Activation of muscarinic receptors leads to rapid activation of several kinases that regulates contraction of longitudinal muscle of colon. PMID: 25891767
9. The relative mRNA expression of M3 and M5 mAChR in bones of a rat osteoporosis model, was investigated. PMID: 24866457
10. The upregulation of M-mAChR during myocardial hypertrophy could relieve the hypertrophic response provoked by angiotensin II. PMID: 24028210
11. Muscarinic M3 receptors are downregulated in the brain stem following spinal cord injury. PMID: 23184186
12. These results suggest a complex antagonistic interplay between G(q)-activated PKC and Gbetagamma in regulation of L-VDCC, in which multiple cytosolic segments of alpha(1C) are involved. PMID: 22990911
13. Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors are the main subtypes expressed in oligodendrocyte progenitor cells. PMID: 21913336
14. Data suggest that Chrm3 is up-regulated in cell membranes of parotid glands in a model of periodontitis and participates in potassium release; Chrm3 expression appears to return to control level upon indomethacin treatment of periodontitis. PMID: 22513211
15. M3 subtype of muscarinic acetylcholine receptor promotes cardioprotection via the suppression of miR-376b-5p PMID: 22396777
16. Taurine supplementation of low-protein diet rats restored Ach-M3R, Synt1, SNAP-25 protein levels and increased sarcoendoplasmatic reticulum Ca2+-ATPase (SERCA) 3 protein expression in rats on a low-protein diet. PMID: 21543213
17. NGF expression increased but M3 subtype muscarinic receptor expression decreased in the seminal vesicle of diabetic rats. PMID: 22141271
18. activation of M3R dimers requires a conformational change of the N-terminal segment of the i3 loop PMID: 22031716
19. structure of the G(q/11)-coupled M3 mAChR ('M3 receptor', from rat) bound to the bronchodilator drug tiotropium and identify the binding mode for this clinically important drug PMID: 22358844
20. Our findings suggest an interaction between vitamin D(3) and muscarinic M3 receptors in regulating insulin secretion from pancreas. PMID: 20655720
21. Expression of muscarinic receptor M1, M3, M5 subunits decreased in the flocculus after unilateral labyrinthectomy. PMID: 19160866
22. study showed that M and M receptors, IP3 and cGMP were functionally regulated during diabetes as function of age PMID: 21054404
23. Chrm3 receptor is located on presynaptic glutamatergic terminals afferent to the recorded pyramidal neuron, thus decreasing glutamate release. PMID: 20600670
24. muscarinic M(1) receptor showed a significant decrease and muscarinic M(3) receptor subtype showed a significant increased binding in the cerebral cortex of hypoglycemic rats compared to diabetic and control PMID: 21126518
25. We found a tendency in the gene expression of muscarinic receptor subtypes from M2 toward M3 after birth trauma. PMID: 20445960
26. NF-kappaB signalling plays an essential role in controlling the expression of the muscarinic M(3) receptor. PMID: 20541544
27. Acetylcholine through muscarinic M1 and M3 receptors stimulated calcium release from the pancreatic islets. PMID: 19554469
28. Increased beta-catenin activity is associated with M(3) mAChR's binding during myocardial infarction. PMID: 19473345
29. Results highlight long-term low dose somatotropin and insulin treatment in regulating muscarinic M1 and M3 cholinergic and NMAR1 and mGluR5 glutamate receptor subtypes in aging rats and rejuvenation of brain function. PMID: 19666081
30. An in situ disulfide cross-linking strategy reveals the structure of the inactive and active state of the M3 muscarinic receptor by location of residues in transmembrane (TM) I close to Cys532, a residue present in the central portion of TM VII. PMID: 12056896
31. in normal bladders both M(2) and M(3) receptors can induce contraction and in the denervated bladder, the M(2) and the M(3) receptors interact in a facilitatory manner to mediate contraction. PMID: 12185001
32. The m1-m5 muscarinic subtypes mapped to the following chromosomes: Chrm1, chromosome 1; Chrm2, chromosome 4; Chrm3, chromosome 17; Chrm4, chromosome 3; and Chrm5, chromosome 3. PMID: 12433399
33. The presence of M3-like receptors on mesencephalic GABAergic neurones was confirmed PMID: 14766941
34. evidence for the presence of M(2)- and M(3)-mAChR, at the mRNA and protein level, in the rat myometrium and that estrogen induces an increase in myometrial responsiveness to mAChR agonists PMID: 15062561
35. central muscarinic M1 and M3 receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in turn control the islet cell proliferation and glucose homeostasis PMID: 15350825
36. data suggest a conformational link between the highly conserved Asp-113, Arg-165, and Tyr-250 residues which is critical for receptor activation PMID: 15572356
37. agonist activation of the M(3) muscarinic acetylcholine receptor results in pronounced conformational changes PMID: 15870064
38. a conformational change occurs adjacent to the ligand-binding pocket of the M(3) muscarinic acetylcholine receptor after agonist binding PMID: 16093246
39. spontaneous contractions in the neonatal rat bladder are enhanced by activation of M2 and M3 receptors PMID: 16709645
40. Interaction of the third intracellular loop of SET protein with M3 muscarinic receptor (M3-MR) leads to reduced M3-MR signaling capacity. PMID: 17065150
41. conclude that previously reported differences relate to the use of inhibitors rather than experimental protocols and that the overall data do not support a role for PLC in M(3) muscarinic receptor-mediated rat bladder contraction PMID: 17596535
42. In reticular thalamic nucleus, the m3-immunolabeling was distributed in a large part of somata and in proximal dendrite shafts than in the distal dendrite region. PMID: 17845913
43. Generation of an agonistic binding site for blockers of the M(3) muscarinic acetylcholine receptor. PMID: 18237275
44. In the inflamed bladder, NO seems to be released via cholinergic stimuli through mucosal muscarinic M3/M5 receptors, presumably on urothelial cells, affecting bladder function PMID: 18246091
45. The muscarinic receptors in the rat gastric mucosal segments were composed of M(1), M(2) and M(3) subtypes PMID: 18938154
46. Taken together, these results suggest that muscarinic M3 receptor is involved in the regulation of glucose uptake and/or lipolysis in adipose tissue. PMID: 19111904
47. Stimulation of the presinaptic M1-muscarinic receptors of the bladder cholinergic nerve terminals and M3-muscarinic receptors located on the bladder detrusor muscle induce the release of urinary bladder-derived relaxant factor. PMID: 19416629
48. results demonstrate that activation of muscarinic receptors exerts diverse effects on GABAergic transmission in the entorhinal cortex PMID: 19494196
49. The muscarinic receptors present in undifferentiated Fisher-rat thyroid epithelial cells belong to the M(3) subtype. Their binding affinities for various antagonists was determined. PMID: 19627722
50. increased PKC-epsilon activity is associated with M3-mAChR during myocardial ischemia PMID: 19685039
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM3 sub-subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:24260

STRING: 10116.ENSRNOP00000067435

UniGene: Rn.87735