Recombinant Rat Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3gat1)

in vitro E.coli expression system

中文名稱：

Recombinant Rat Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1(B3gat1)
貨號：

CSB-CF002496RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

基因名：

B3gat1
Uniprot No.：

O35789
別名：

B3gat1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-334
氨基酸序列

MPKRRDILAIVLIVLPWTLLITVWHQSSLAPLLAVHKDEGSDPRHEAPPGADPREYCMSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSTQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
基因功能參考文獻(xiàn)：
1. positive expression for Osterix and HNK1 in undifferentiated ectomesenchymal cells in early and late stages PMID: 26253417
2. increased expression of CS-GAGs and GlcAT-I in fibrotic lungs and fibrotic lung fibroblasts PMID: 21056957
3. Results of these studies demonstrate that BMP-2 and TGF-beta regulate GlcAT-1 expression in nucleus pulposus cells through a signaling network comprising MAPK, AP1, Sp1, and TonEBP PMID: 19961337
4. These results combined suggest that both PSA and HNK-1 carbohydrate epitopes are synthesized and may have an important role in the adult peripheral vestibular endorgans. PMID: 15232286
5. Trifluralin-induced total hepatic UGT enzymes (2.4-fold) and mRNA expression of selected hepatic UGT isozymes (UGT1A1, 1.4-fold; UGT1A6, 6.4-fold; UGT2B1, 3.7-fold). PMID: 18582544
6. TonEBP activation by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral disc PMID: 19147493
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亞細(xì)胞定位：

[Isoform 1]: Golgi apparatus membrane; Single-pass type II membrane protein. Secreted.; [Isoform 2]: Golgi apparatus membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Secreted.
蛋白家族：

Glycosyltransferase 43 family
組織特異性：

Brain. Greater expression found in the cerebral cortex than the cerebellum.
數(shù)據(jù)庫鏈接：

KEGG: rno:117108

STRING: 10116.ENSRNOP00000009825

UniGene: Rn.88274