Recombinant Human Proteinase-activated receptor 2 (F2RL1)
產(chǎn)品詳情
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基因名：
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Uniprot No.：
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別名：F2RL1; GPR11; PAR2; Proteinase-activated receptor 2; PAR-2; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; Thrombin receptor-like 1
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種屬：Homo sapiens (Human)
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蛋白長度：Full Length of Mature Protein
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表達區(qū)域：37-397aa
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氨基酸序列SLIGKVDGTSHVTGKGVTVETVFSVDEFSASVLTGKLTTVFLPIVYTIVFVVGLPSNGMALWVFLFRTKKKHPAVIYMANLALADLLSVIWFPLKIAYHIHGNNWIYGEALCNVLIGFFYGNMYCSILFMTCLSVQRYWVIVNPMGHSRKKANIAIGISLAIWLLILLVTIPLYVVKQTIFIPALNITTCHDVLPEQLLVGDMFNYFLSLAIGVFLFPAFLTASAYVLMIRMLRSSAMDENSEKKRKRAIKLIVTVLAMYLICFTPSNLLLVVHYFLIKSQGQSHVYALYIVALCLSTLNSCIDPFVYYFVSHDFRDHAKNALLCRSVRTVKQMQVSLTSKKHSRKSSSYSSSSTTVKTSY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering. -
蛋白標簽：N-terminal 10xHis-tagged
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產(chǎn)品提供形式：Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液：Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet & COA：Please contact us to get it.
產(chǎn)品評價
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靶點詳情
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功能：Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2R/PAR1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as GNAQ, GNA11, GNA14, GNA12 and GNA13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. However, according to PubMed:21627585 can signal through G(i) subunit alpha. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to GNAQ and GNA11; the function involves dissociation of RIPK1 and TRADD from TNFR1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'; the function involves IKBKB and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of GNAQ and GNA11 and involves promotion of cofilin dephosphorylation and actin filament severing. Induces redistribution of COPS5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by COPS5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells; activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses. Implicated in a number of acute and chronic inflammatory diseases such as of the joints, lungs, brain, gastrointestinal tract, periodontium, skin, and vascular systems, and in autoimmune disorders.
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基因功能參考文獻：
- Study findings point out that PAR2 could play an essential role in gastroesophageal reflux disease (GERD) pathogenesis - even repeated short-term exposure to weakly acidic conditions lead to the upregulation of PAR2 and subsequent activation of the intense IL-8 release in the esophageal mucosa and initiation of mucosal immune response in GERD. PMID: 29672302
- PAR-2 is expressed basolaterally in airway cells, where it stimulates both intracellular Ca(2+) release and Ca(2+) influx, which activates low-level nitric oxide production, increases apical membrane Cl(-) permeability approximately 3-5-fold, and increases ciliary beating approximately 20-50%. PMID: 28874459
- PAR2 signaling from endosomes underlies the persistent hyperexcitability of nociceptors that mediates chronic pain of irritable bowel syndrome. PMID: 30012612
- PAR2 plays an important role in the proliferation and metastasis of hepatocellular carcinoma. PMID: 29563756
- PAR2 expression is crucial for TGF-beta1-induced ERK activation and cell motility. Functional cooperation of PAR2 and TGF-beta1 involves a physical interaction between PAR2 and ALK5. PMID: 29261154
- Activation of PAR2 inhibits the expression of IL-10 in B cells, which can be reversed by treating B cells with Bcl2L12 shRNA-carrying liposomes. PMID: 28426164
- High Expressions of PAR2 is associated with cancer. PMID: 28418856
- activation of PAR2 compromises the vascular endothelial barrier function by suppressing the expression of Ve-cadherin. PMID: 28485540
- plays a direct role in melanogenesis by increasing stem cell factor secretion from keratinocytes PMID: 26909822
- Neutrophil elastase enhances IL-12p40 production by lipopolysaccharide-stimulated macrophages via transactivation of the PAR-2/EGFR/TLR4 signaling pathway PMID: 27282560
- PAR2 is crucial for TGF-beta1-induced cell motility by its ability to sustain expression of ALK5. Therapeutically targeting PAR2 may thus be a promising approach in preventing TGF-beta-dependent driven metastatic dissemination in PDAC and possibly other stroma-rich tumour types. PMID: 27248167
- Protease-activated receptor 2 (PAR2) is present in human skin. PMID: 27981668
- PAR2 signaling promotes cancer cell migration through miR-205/BMPR1B pathway in human colorectal carcinoma. PMID: 28990808
- findings showed in intestinal epithelial cells that PAR-2 activation leads to polarized IL-8 secretion in accordance with the side of PAR-2 activation, apical or basolateral, but do not affect ubiquitin proteasome system; demonstrate that PAR-2 activation leads to an increased IL-8 production and can affect proteasome system, particularly when PAR-2 activation was induced in the apical side PMID: 27455449
- TF-induced microvessel stabilization is regulated via PAR2-SMAD3 that is indispensable for the maintenance of vascular integrity. PMID: 26658897
- PAR-2- and PAR-1-mediated TNF-alpha release from monocytes suggests that these unique protease receptors are involved in the pathogenesis of inflammation. PMID: 27423452
- Results indicate that chymotrypsin-like serine protease enhances soluble fms-like tyrosine kinase 1 production through protease-activated receptor-2 in trophoblast cells and thus plays an important additional role in preeclampsia pathogenesis. PMID: 27140908
- crystal structures of PAR2 in complex with two distinct antagonists and a blocking antibody PMID: 28445455
- PAR-2 plays an important role in the progression of ovarian clear cell carcinoma. PMID: 28438620
- Data suggest activation of PAR2 via FVIIA/TF signaling activates PI3K/AKT signaling, inactivates GSK3b signaling, leads to accumulation of beta-catenin, and promotes tumor cell migration/invasion. (PAR2 = protease-activated receptor 2; FVIIA = coagulation factor VIIa; TF = tissue factor/thromboplastin; PI3K = phosphatidylinositol 3-kinase; AKT = proto-oncogene protein c-akt; GSK3b = glycogen synthase kinase 3 beta) PMID: 28522609
- PAR2 modulation was sufficient to induce islet cell transdifferentiation in the absence of beta-cells. PMID: 27809303
- Studies provide increasing evidence that PAR2 plays a significant role in inflammatory diseases both in the periphery and in the CNS. There is a clear similarity between PAR2 expression and activation on cells of the immune system and those cell types that are proposed to play a role within the CNS, astrocytes and microglia. [review] PMID: 26648078
- Results show that PAR2 in hepatic stellate cells plays a crucial role in promoting hepatocellular carcinoma growth presumably by mediating migration and secretion of pro-angiogenic and pro-mitotic factors. PMID: 27473374
- Increase in airway smooth muscle mass, possibly involving aberrant expression and activation of PAR-2-mediated pathways, characterizes younger patients with severe asthma with poor asthma control PMID: 27001157
- PAR2/GSK3beta is a novel pathway that plays a critical role in the regulation of stem/progenitor cell survival and proliferation in normal colon crypts and colon cancer. PMID: 27313176
- Study suggests that deregulated pericellular matriptase activity in OSCC may transactivate PAR-2 on fibroblasts in the surrounding tissue and thus promote their recruitment to the perimeter of the tumor, contributing to a microenvironement that favors tumor growth of oral squamous cell carcinoma. PMID: 27615543
- Study demonstrates that AHRR and F2RL3 methylation are strong predictors individually for lung cancer development. PMID: 27924164
- PAR2 activation promoted obesity. PMID: 27006943
- involved in pathway induced by house dust mites, which has anti-apoptotic effects on neutrophils of normal and allergic subjects PMID: 26666432
- Activation of PAR-2 by SLIGRL, a PAR-2 specific ligand, leads to dual phosphorylation of both catalytic sites but primarily regulated eNOS-Thr-495 phosphorylation with no change in nitric oxide production. PMID: 26729042
- Stimulation of PAR-2 activates Nf-kappaB signaling, resulting in RelA nuclear translocation and enhanced expression of pro-inflammatory mRNAs in oral squamous cell carcinoma. PMID: 26839311
- This study provides evidence for the major role of PAR-2 in the pathogenesis of gastroesophageal reflux disease (GERD) and GERD-associated mucosal alterations. PMID: 25265427
- PAR-2 expression is increased on CD14++CD16+ monocytes in the peripheral blood of subjects with severe asthma and may be a biomarker of asthma severity. PMID: 26658828
- PAR-2 contributes to the progression of pulmonary fibrosis, and targeting PAR-2 may be a promising therapeutic strategy for treating pulmonary fibrosis. PMID: 26147947
- Binding of the PH domain of PAR-2 enables breast cancer growth and extravillous trophoblast invasion. PMID: 26600192
- Results show that exposure of bronchial epithelial cells to Aspergillus fumigatus extract suppressed poly I:C and HRV16 signaling via a mechanism shown to involve activation of PAR-2 and PTPN11. PMID: 26072921
- alpha-Enolase increases after injury and may activate pulmonary endothelial cells and prime PMNs through plasmin activity and PAR-2 activation PMID: 25944790
- Study showed that PAR-2, in CSF, was not specifically altered in prion diseases compared to other neurodegenerative conditions. PMID: 25886404
- HAI-1 may have a critical role in maintaining normal keratinocyte morphology through regulation of PAR-2-dependent p38 mitogen-activated protein kinase signaling. PMID: 25842366
- In colorectal cancer, miR-125b mediates PAR2-induced cancer cell migration by targeting Gab2. PMID: 26354435
- PAR-2-mediated IL-13 production by human monocytes may be dependent on the Ca2+/ERK2 signaling pathway. PMID: 25976462
- Coculture of human cancer cells with murine trigeminal neurons demonstrated colocalization of TMPRSS2 with PAR2. PMID: 25734995
- These findings suggested that the pathophysiology of heartburn sensation or esophageal hypersensitivity may be associated with the activation of PAR-2, TRPV1, and acid-sensing ion channels. PMID: 26294672
- GM-CSF primes IL-13 production by macrophages via PAR-2. PMID: 25633855
- Data indicate that PAR2 stimulation induces the production of many key inflammatory mediators. PMID: 26238490
- PAR2 up regulated in sebaceous lipogenesis, inflammation and innate immunity. PMID: 25880702
- Serine protease activity of Per a 10 activates A549 cells to secrete proinflammatory cytokines by PAR-2 activation and Ca(2+)mobilization and can be exploited therapeutically. PMID: 25468564
- Cytokine induction by diesel exhaust particles is suppressed by PAR-2 silencing in bronchial epithelial cells. PMID: 26160521
- PAR-2 regulates the growth and invasion of oral squamous carcinoma cells. PMID: 25912757
- PAR2 and TRPV4 are activated by neutrophil elastase to cause inflammation and pain PMID: 25878251
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亞細胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family
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組織特異性：Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. Expressed in endothelial cells.
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