Recombinant Human Potassium channel subfamily K member 9 (KCNK9)

中文名稱：

人KCNK9重組蛋白
貨號：

CSB-CF889068HU
說明書：

MSDS Datasheet
規(guī)格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

KCNK9
Uniprot No.：

Q9NPC2
別名：

(Acid-sensitive potassium channel protein TASK-3)(TWIK-related acid-sensitive K(+) channel 3)(Two pore potassium channel KT3.2)(Two pore K(+) channel KT3.2)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

48.3 kDa
表達(dá)區(qū)域：

1-374aa
氨基酸序列

MKRQNVRTLSLIVCTFTYLLVGAAVFDALESDHEMREEEKLKAEEIRIKGKYNISSEDYRQLELVILQSEPHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLLKRIKKCCGMRNTDVSMENMVTVGFFSCMGTLCIGAAAFSQCEEWSFFHAYYYCFITLTTIGFGDYVALQTKGALQKKPLYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNSEDERRDAEERASLAGNRNSMVIHIPEEPRPSRPRYKADVPDLQSVCSCTCYRSQDYGGRSVAPQNSFSAKLAPHYFHSISYKIEEISPSTLKNSLFPSPISSISPGLHSFTDHQRLMKRRKSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

pH-dependent, voltage-insensitive, background potassium channel protein.
基因功能參考文獻(xiàn)：
1. observations implicate TASK-3 as a critical factor in cell cycle progression and corroborate its potential as a therapeutic target in breast cancer treatment. PMID: 29596383
2. point mutations in DLK1 and KCNK9 at least do not seem to be a common cause of central precocious puberty in girls. PMID: 28672280
3. In conclusion, we have identified the first selective activator of the two-pore domain potassium channel TASK3. PMID: 28882594
4. KCNK9 imprinting syndrome is a rare cause of intellectual disability, congenital hypotonia, palatal abnormalities, and occasional seizures. PMID: 27151206
5. Our results provide evidence for new loci influencing abdominal visceral (BBS9, ADCY8, KCNK9) and subcutaneous (MLLT10/DNAJC1/EBLN1) fat, and confirmed a locus (THNSL2) previously reported to be associated with abdominal fat in women. PMID: 26480920
6. TASK-1 and TASK-3 may form heterodimers in human atrial cardiomyocytes. PMID: 25655935
7. During conductance simulation experiments, both TASK-3 and TREK-1 channels were able to repolarise the membrane once AP threshold was reached PMID: 25482670
8. The findings of this study suggest that variations in KCNK9 genes are associated with both mild and severe persistent breast pain after breast cancer surgery. PMID: 25599232
9. Diacylglycerol mediates regulation of TASK1 and TASK3 potassium channels by GNAQ. PMID: 25420509
10. Interference with TASK-3 channel expression, therefore, induces caspase-dependent and -independent apoptosis of melanoma cells, most likely via causing mitochondrial depolarization. PMID: 25318378
11. K2P3.1 and K2P9.1 undergo rapid dynamin-dependent endocytosis PMID: 23807092
12. Mutations of KCNK9 or epigenetic disturbances within the PEG13 imprinted cluster do not significantly contribute to the cause of the developmental disabilities tested in this study. PMID: 24980697
13. Two SNPs replicated: the paternal rs2471083-C allele (located near the imprinted KCNK9 gene) and the paternal rs3091869-T allele (located near the SLC2A10 gene) increased BMI equally (beta = 0.11 (SD), P<0.0027) PMID: 25078964
14. For KCNK9, two SNPs (i.e., rs3780039, rs11166921) were associated with the occurrence of preoperative breast pain. PMID: 24392765
15. We observed co-localization of the TASK-3 protein and a mitochondrial marker in the mitochondria of HaCaT cells. PMID: 24126847
16. By both a gain-of-function additional mutation of TASK3 channels. PMID: 24342771
17. TNFalpha activation and TASK3 channel activity can promote cellular apoptosis. PMID: 24307172
18. Overexpression of TASK-3 expression is associated with ovarian cancer. PMID: 23564779
19. Results of an exploratory study suggest that blood pressure and aldosterone production may be affected by variations in KCNK9. The findings could have relevance to risk for hypertension. PMID: 22893713
20. an increase in TASK-3 expression levels reduces cell migration/invasion in breast cancer cells PMID: 21910834
21. Depletion of the endogenous phosphatidylinositol-4,5-bisphosphate pool in living cells consistently finds no change in currents mediated by TASK-1 and TASK-3. PMID: 21540350
22. TASK-3 immunoreactivity was detected in both astrocytes and microglia of temporal lobe epilepsy patients. PMID: 21710317
23. TASK-3 channels are functionally present in the mitochondria of the melanoma cells, and their function is essential for the survival of these cells. PMID: 21512417
24. cAMP-dependent protein kinase is responsible for the phosphorylation of the terminal serine in both K(2P)3.1 and K(2P)9.1 PMID: 21357689
25. Western analysis confirms expression of TASK1 and TASK3 in medulloblastoma cells. PMID: 20931182
26. KCNK9 gene is imprinted in human and mouse, exhibiting preferential expression from the maternal allele in brain. PMID: 17704508
27. TASK-1 and 3 are determinant of aldosterone secretion and adrenocortical zonation. PMID: 20049674
28. TASK-3 responds to voltage in a way that reveals gating at its inner, cytoplasmic mouth through movements of membrane helices M2 and M4. PMID: 19703964
29. TASK-1 and TASK-3 differed insofar as a large portion of the C terminus was necessary for the full effects of halothane and TRH on TASK-3 but not on TASK-1 PMID: 11886861
30. Overexpression of KCNK9 in cell lines promotes tumor formation and confers resistance to both hypoxia and serum deprivation. PMID: 12676587
31. results establish a direct link between the potassium channel activity of TASK3 and its oncogenic functions and imply that blockers for this potassium channel may have therapeutic potential for the treatment of cancers PMID: 12782791
32. TASK3 is expressed in pia mater, astrocytes, Purkinje and granule cells PMID: 15197476
33. TASK-3 channels underlie a major component of cerebellar granule neurons leak current which is sensitive to block by zinc PMID: 15284350
34. Overexpression rather than mutation of the KCNK9 gene may contribute to the development of colorectal cancers. PMID: 15601307
35. The expression pattern of TASK-3 in Melanoma cells is determined. PMID: 17013562
36. (Glutamic acid-aspartic acid-glutamic acid) in human TASK-3 is a major determinant of the rate of endoplasmic reticulum export and is required for efficient surface expression of the channel. PMID: 17547699
37. TASK-3 channels are present in the mitochondria in both malignantly transformed and healthy cells, suggesting that they might have roles in ensuring mitochondrial functions. PMID: 18094996
38. TASK3 channels may possibly represent a novel molecular target for the treatment of human glioblastoma PMID: 18217213
39. K(2)P channels as novel potassium conductance on T lymphocytes critically influencing T cell effector function and identify a possible molecular target for immunomodulation in T cell-mediated autoimmune disorders PMID: 18375952
40. the TASK3 M1P1 loop lies close to the pore, regulating TASK3 channel activity PMID: 18417474
41. a mutation in the genomically imprinted potassium channel KCNK9 may have a role in the maternally inherited Birk Barel mental retardation dysmorphism syndrome PMID: 18678320
42. Thus, regulated expression of TASK channels might contribute to a molecular switch between death and survival of neurons in autoimmune CNS inflammation. PMID: 18824070
43. Both TASK-3 and TREK-1 are functionally operational in the adrenocortical H295R cell line, modulate membrane potential and aldosterone secretion. PMID: 18854423
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相關(guān)疾病：

Birk-Barel mental retardation dysmorphism syndrome (BIBAS)
亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

Two pore domain potassium channel (TC 1.A.1.8) family
組織特異性：

Mainly found in the cerebellum. Also found in adrenal gland, kidney and lung.
數(shù)據(jù)庫鏈接：

HGNC: 6283

OMIM: 605874

KEGG: hsa:51305

STRING: 9606.ENSP00000302166

UniGene: Hs.493037