Recombinant Human Neurotensin receptor type 1 (NTSR1)

中文名稱：

Recombinant Human Neurotensin receptor type 1 (NTSR1)
貨號：

CSB-CF016137HU
說明書：

MSDS Datasheet
規格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

NTSR1
Uniprot No.：

P30989
別名：

NT-R-1;NTR1;High-affinity levocabastine-insensitive neurotensin receptor;NTRH
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

47.8 kDa
表達區域：

1-418aa
氨基酸序列

MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

G-protein coupled receptor for the tridecapeptide neurotensin (NTS). Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis.
基因功能參考文獻：
1. NTSR1 knockdown impaired glioblastoma invasion and tumor growth through miR-494 and identified a novel mechanism by which siNTSR1 suppressed glioblastoma invasion through the miR-494/SOCS6 axis in glioblastoma. PMID: 30261490
2. This study strongly suggests that the addition of NTSR1 inhibitor in combination with platinum salt-based therapy will improve the response to the drug. PMID: 28790113
3. Patients with lung cancer have specific personality traits, and variations in the neurotensin receptor 1 gene may be involved in the biological mechanisms of the harm avoidance and reward dependence personality traits. PMID: 27891817
4. Our findings identify promoter methylation as an important process regulating the differential expression or silencing of NTSR1/2 in Colorectal cancer (CRC) cells. Moreover, inhibition of NTSR1 repressed tumorigenic effects in CRC cells, suggesting that NTSR1 may be used as a therapeutic target for CRC. PMID: 28498396
5. inducing exogenous neurotensin stimulation and enhancing NTR1 expression promoted tumor invasion rather than proliferation by accelerating epithelial-to-mesenchymal transition in hepatocellular carcinoma cells. PMID: 27611941
6. High NTR1 expression is associated with pancreatic ductal adenocarcinoma. PMID: 28536844
7. Data suggest that small molecule ligand binding to neurotensin receptor 1 (NTSR1) could facilitate the structure-based design of non-peptide ligands for the evaluation of the pharmacological potential of NTSR1 in neurological disorders and cancer. PMID: 27491650
8. The results reveal a positive feedback loop between NTS/NTSR1 and Wnt/beta-Catenin signaling in glioblastoma cells that might be important for tumor development and provide potential therapeutic targets for glioblastoma. PMID: 29065410
9. NTSR1 overexpression is a poor prognostic factor in endometrial cancer, highlighting the contribution of NTS in endometrial cancer progression PMID: 28836043
10. characterization of the hNTS1-mediated cellular signaling network PMID: 28341345
11. Heterodimerization of the kappa opioid receptor and neurotensin receptor 1 contributes to a novel beta-arrestin-2-signaling pathway. PMID: 27523794
12. Silencing of miR-133alpha or overexpression of aftiphilin attenuated NTR1 trafficking to plasma membrane in human colonocytes. PMID: 26902265
13. Neurotensin receptor-targeted oncolytic adenovirus co-expressing decorin and Wnt antagonist useful for systemic treatment of NTR-overexpressing pancreatic cancer. PMID: 26471393
14. high expression of NTSR1 is found in clinical NETs and promoter methylation is an important mechanism controlling the differential expression of NTSR1 and silencing of NTSR2 in NET cells PMID: 26298774
15. Data suggest that neurotensin receptor 1 (NTSR1) methylation may be a prognostic biomarker in colorectal cancers (CRCs). PMID: 26334593
16. The presence of F358(7.42) causes the conserved W321(6.48) to adopt side chain orientation parallel to the lipid bilayer sealing the collapsed Na(+) ion pocket and linking the agonist with residues in the lower receptor part implicated in GPCR activation. PMID: 26205105
17. This study indicated that NTR1 may play an important role in tumor progression of gastric cancer PMID: 26215716
18. High expression levels of NTS and NTSR1 predict a poor prognosis in glioma patients. PMID: 25644759
19. Neurotensin receptor 1 SNPs were significantly associated with processing speed in a sample of Chinese college students. PMID: 25159184
20. the NTR1-HIF-1alpha-VEGFalpha axis has a role in intestinal angiogenic responses and in the pathophysiology of colitis and inflammatory bowel disease PMID: 25307345
21. This review provides a summary of current knowledge, mainly emerging from microdialysis studies, supporting the presence of a neurotensin receptor 1/D2 receptor heteromer in the brain. [review] PMID: 25175457
22. Taken together, our results provided a novel regulatory mechanism for GPR39-1b in NTRS1 signaling. PMID: 24512471
23. variations in the NTR1 gene were involved in the biological mechanisms of HA and RD personality traits; however, the effect is influenced by gender. PMID: 24401289
24. The current study investigated whether genetic polymorphisms in the NTR1 gene (rs6090453C/G, rs6011914C/G, and rs2427422A/G) were associated with the performance on verbal and visual learning. PMID: 24770449
25. NTSR1 is commonly high expressed in melanoma cells. PMID: 24357116
26. NTSR1 gene variants are associated with alcohol dependence in a male Han Chinese population. PMID: 23743782
27. The association between NTR1 gene single nucleotide polymorphisms (SNPs) (rs6090453, rs6011914, and rs2427422) and coping styles, were evaluated. PMID: 23807075
28. NTSR1 in colonic epithelial cells is overexpressed in inflammatory bowel disease, in a stepwise fashion with sequential progress from inflammation to dysplasia and carcinoma. PMID: 23901225
29. rs6090453C/G polymorphism and the CGG haplotype may enhance schizophrenia susceptibility in the Han Chinese population, while the GCG haplotype may be a protective factor, particularly in females. PMID: 23483448
30. The study found no evidence for the possible association between three NTR1 SNPs and both trait and state anxiety. PMID: 23292156
31. These results indicate that the association between hippocampal structure and working memory performance was modulated by variation in the NTSR1 gene PMID: 23110888
32. Data indicate that dopamine D2 receptor (D2R) and neurotensin 1 receptor (NTS1R) were colocated in the plasma membrane of cells. PMID: 23624386
33. analysis of the role of cholesterol on the activity and stability of neurotensin receptor 1 PMID: 22551944
34. Integrin alpha(nu) beta(3), NTRS1 and PSCA mRNA expression increased with tumorigenic potential, but mRNA expression levels for these proteins do not translate directly to equivalent expression levels of membrane bound protein. PMID: 21748756
35. Our studies demonstrate that NTSR-1 plamitoylation is required for NTSR-1-mediated MAPK signaling and cellular proliferation in breast cancer cells. PMID: 21725197
36. Neurotensin receptor 1 is expressed in gastrointestinal stromal tumors but not in interstitial cells of Cajal. PMID: 21364741
37. NTSR1 single nucleotide polymorphisms were significantly associated with variance in working memory performance among healthy adults. PMID: 21394204
38. Four splice variants of the NTS1 receptor were detected in prostate cancer cell lines. These isoforms are expressed in the prostate cancer cell lines PC3 and DU145, but not in LNCaP or in normal prostate tissue, which only express the normal transcript. PMID: 20018219
39. this study proposes a novel function of NTR2 in the regulation of NTR1 activity. PMID: 19968961
40. NTR1 was upregulated in cells with a basal phenotype (cytokeratin 1/5/10/14+) PMID: 20048080
41. investigation of its binding to neurotensin PMID: 11906607
42. Neurotensin receptor-1 and -3 complex modulates the cellular signaling of neurotensin in the HT29 cell line. PMID: 12360476
43. constant activation of NT1 receptor generates an oncogenic regulation PMID: 14699144
44. neuropeptide neurotensin (NT) binds to freshly isolated Sezary malignant cells and induces through NT1 receptors the cell migration of the cutaneous T cell lymphoma cell line Cou-L. PMID: 14962098
45. NT and NTR1 are part of network activated after mucosal injuries, and NT stimulates epithelial restitution, at least in part, through a COX-2 dependent pathway. PMID: 15764810
46. this report establishs a novel link in vitro between the Tcf/beta-catenin pathway and NT1 receptor promoter activation PMID: 16299383
47. NMU & its cancer-specific receptors NTSR1 & GHSR1b, as well as its target genes, are overexpressed in lung cancer and in cell lines, and that those gene products play indispensable roles in the growth and progression of lung cancer cells. PMID: 17018595
48. Binding of NTSR1 in a prostatic neoplasm cell line is sensitive to metabolic stress. PMID: 17289170
49. results suggest that increased NTSR1 expression may be an early event during colonic tumorigenesis and also contribute to tumor progression and aggressive behavior in colonic adenocarcinomas. PMID: 18541341
50. NTR1 and NTR2 mRNA were not detected in either pituitary adenomas or normal tissue. PMID: 18624930
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Membrane raft.
蛋白家族：

G-protein coupled receptor 1 family, Neurotensin receptor subfamily, NTSR1 sub-subfamily
組織特異性：

Expressed in prostate (at protein level). Detected in colon and peripheral blood mononuclear cells. Detected at very low levels in brain.
數據庫鏈接：

HGNC: 8039

OMIM: 162651

KEGG: hsa:4923

STRING: 9606.ENSP00000359532

UniGene: Hs.590869