Recombinant Alpha-hemolysin translocation ATP-binding protein HlyB (hlyB)

in vitro E.coli expression system

中文名稱：

大腸桿菌O157：H7hlyB重組蛋白
貨號：

CSB-CF673857EOD
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

hlyB
Uniprot No.：

Q46717
別名：

hlyB; L7049; ECO57PM18; Alpha-hemolysin translocation ATP-binding protein HlyB; EHEC-HlyB protein
種屬：

Escherichia coli O157:H7
蛋白長度：

full length protein
表達區域：

1-706
氨基酸序列

MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVTEWLLAAKSIGLKAKYV EKHFSRLSIISLPALIWRDDGKHYILSRITKDSSRYLVYDPEQHQSLTFSRDEFEKLYQG KVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTVSAFIQFLALITPLFFQVVMD KVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSHTTSRIDVELGAKLFRHLLALPV SYFENRRVGETVARVRELEQIRNFLTGQALTSVLDLFFSVIFFCVMWYYSPQLTLVILLS LPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMAVSPQMIATWDKQL AGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEISIGQLIAFNMLAGQV IAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPEIQGDIEFKNVRFRYSSD GNVILNNINLYISKGDVIGIVGRSGSGKSTLTKLLQRFYIPETGQILIDGHDLSLADPEW LRRQIGVVLQENILLNRSIIDNITLASPAVSMEQAIEAARLAGAHDFIRELKEGYNTIVG EQGVGLSGGQRQRIAIARALVTNPRILIFDEATSALDYESENIIMKNMSRICKNRTVIII AHRLSTVKNANRIIVMDNGFISEDGTHKELISKKDSLYAYLYQLQA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Part of the ABC transporter complex HlyBD involved in hemolysin export. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
基因功能參考文獻：
1. H662 acts as a 'linchpin', holding together all required parts of a complicated network of interactions between ATP, water molecules, Mg2+, & amino acids both in cis & trans, necessary for intermonomer communication. PMID: 15889153
2. Principles of how ATP-hydrolysis is coupled to energy transduction, including the conformational changes occurring during the catalytic cycle, leading to substrate transport. (review) PMID: 16246029
亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

ABC transporter superfamily, Protein-1 exporter (TC 3.A.1.109) family
數據庫鏈接：

KEGG: ece:Z_L7049

STRING: 155864.L7049