V5-Tag Monoclonal Antibody
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中文名稱：V5-Tag鼠單克隆抗體
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貨號：CSB-MA000161M0m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: 50ng V5-tagged fusion protein
V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 37.8 KDa
Observed band size: 43 KDa
Exposure time: 10S -
Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000
Lane 1: Recombinant V5-tagged fusion protein at 50ng
Lane 2: Recombinant V5-tagged fusion protein at 25ng
Lane 3: Recombinant V5-tagged fusion protein at 12.25ng
Lane 4: Recombinant V5-tagged fusion protein at 6.25ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 37.8 kd
Observed band size: 43 kd
Exposure time: 5min -
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa
Observed band size: 52, 47, 67, 42 KDa
Exposure time: 30s -
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa
Observed band size: 32, 21, 28, 58 KDa
Exposure time: 5min -
Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L). -
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG1 instead of CSB-MA000161M0m in Transfected HEK293F cells whole cell lysate
Lane 2: CSB-MA000161M0m (2.5μl) + Transfected HEK293F cells whole cell lysate (500μg)
Lane 3: Transfected HEK293F cells whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000161M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 -
Overlay histogram showing untransfected HEK293F cells (green line) or transfected HEK293F cells (red line) stained with CSB-MA000161M0m. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：V5-Tag單克隆抗體（CUSABIO貨號：CSB-MA000161M0m）是針對人工合成V5表位標簽開發的高特異性科研工具，該標簽廣泛應用于重組蛋白追蹤、純化及功能驗證領域。本產品采用單克隆抗體技術制備，具有廣譜物種兼容性，可識別融合表達于目標蛋白N端或C端的V5標簽序列，適用于Western Blot、免疫熒光染色、免疫共沉淀及流式細胞術等多重檢測平臺，實驗數據穩定可靠。在蛋白表達驗證、亞細胞定位分析、蛋白互作研究等場景中表現優異，尤其適合需要高靈敏度檢測的哺乳動物表達系統研究。該抗體經嚴格質控驗證，批次一致性優異，1:1000推薦稀釋比可滿足多數實驗體系需求，為基因編輯驗證、信號通路機制探索及蛋白組學研究提供高效解決方案。其多平臺兼容特性顯著提升實驗設計靈活性，是分子生物學、細胞生物學領域研究者的優選試劑。
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基因名：V5-Tag
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宿主：Mouse
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反應種屬：All
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免疫原：GKPIPNPLLGLDST synthetic peptide conjugate to KLH
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號：8A7A8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:2000-1:80000 IF 1:100-1:200 IP 2μl-5μl FC 1:100-1:300 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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