GST Monoclonal Antibody
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中文名稱：GST鼠單克隆抗體
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貨號：CSB-MA000031M1m
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規(guī)格：￥400
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圖片：
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Western Blot
Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli
All lanes: GST antibody at 1:10000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa
Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa
Exposure time：5min -
Western Blot
Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli
All lanes: GST antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 26, 102.9, 75.6 KDa
Observed band size: 26, 103, 76 KDa
Exposure time：1min -
Western Blot
Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: GST antibody at 1:10000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 26 KDa
Observed band size: 26 KDa
Exposure time：5min -
Western Blot
Positive WB detected in: 25ng recombinant protein with GST tag from E.coli
GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 26 KDa
Observed band size: 26 KDa
Exposure time：5min -
Immunofluorescence staining of 293F transfected cells with CSB-MA000031M1m at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). -
Immunoprecipitating GST in transfected-293F whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA000031M1m in 293F cell lysate Transfected with GST
Lane 2: CSB-MA000031M1m (5μg) + Transfected-293F whole cell lysate (500μg)
Lane 3: Transfected-293F whole cell lysate (10μg)
For western blotting, the blot was detected with CSB-MA000031M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 -
Overlay Peak curve showing 293F cells transfected with GST stained with CSB-MA000031M1m (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：本產(chǎn)品為高特異性GST單克隆抗體（CUSABIO貨號：CSB-MA000031M1m），靶向谷胱甘肽S-轉移酶（GST），該蛋白作為重要的Ⅱ相代謝酶，在細胞解毒、藥物代謝及氧化應激調(diào)控等研究中具有關鍵作用。該抗體具有廣譜物種反應性，適用于ELISA、Western Blot、免疫熒光、流式細胞術及免疫共沉淀等多種實驗體系，可精準識別天然及重組GST蛋白。其高親和力特性在重組蛋白純化驗證、GST融合蛋白相互作用分析、代謝通路研究等科研場景中表現(xiàn)優(yōu)異，特別適用于藥物開發(fā)中代謝酶活性檢測及毒理學研究。經(jīng)嚴格質控驗證，該抗體在多種實驗條件下均保持穩(wěn)定性能，為腫瘤機制探索、環(huán)境毒物代謝分析等基礎研究提供可靠工具。作為科研專用試劑，本產(chǎn)品廣泛應用于分子生物學、細胞生物學及生物化學領域，是研究蛋白質相互作用網(wǎng)絡和代謝調(diào)控機制的重要工具抗體。
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基因名：GST
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別名：Glutathione S-Transferase, gst, GST tag
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宿主：Mouse
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反應種屬：All
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免疫原：Recombinant GST Protein
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG2b
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純化方式：>95%, Protein A purified
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克隆號：3D3E4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, WB, IF, FC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:10000-1:640000 IF 1:100-1:300 FC 1:100-1:300 IP 1ul-5ul -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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