GFP Monoclonal Antibody
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中文名稱：GFP鼠單克隆抗體
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貨號：CSB-MA000051M1m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: 50ng recombinant protein
All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min -
Western Blot
Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng
All lanes: GFP antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time:5min -
Western Blot
Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg
All lanes GFP antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
Observed band size: 32,32,50,32,32 KDa
Exposure time:1min -
Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
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Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
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Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP
Lane 1: Mouse control IgG2b instead of CSB-MA000051M1m in 293F whole cell lysate transfected with GFP
Lane 2: CSB-MA000051M1m (4μg) + 293F whole cell lysate transfected with GFP (500μg)
Lane 3: 293F whole cell lysate transfected with GFP (5μg)
For western blotting, the blot was detected with CSB-MA000051M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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其他：
產品詳情
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產品描述：本產品為GFP單克隆抗體（CUSABIO貨號：CSB-MA000051M1m），專為檢測綠色熒光蛋白（GFP）設計。GFP作為分子生物學中廣泛應用的熒光標記蛋白，其特異性檢測在基因表達示蹤、蛋白定位分析及細胞功能研究中具有重要價值。該抗體通過ELISA、Western Blot（WB）、免疫熒光（IF）、流式細胞術（FC）和免疫沉淀（IP）五大實驗平臺驗證，適用于轉基因細胞株篩選、重組蛋白表達驗證、熒光融合蛋白追蹤等科研場景，展現廣泛的物種兼容性。其高靈敏度和特異性可精準識別天然GFP及其衍生變體（如EGFP、YFP），在活細胞成像、蛋白質相互作用研究及報告基因系統中表現卓越。作為科研級試劑，本產品采用高純度雜交瘤技術制備，批次穩定性優異，為熒光標記實驗提供可靠工具，助力基因編輯、細胞信號通路及亞細胞結構研究。核心關鍵詞：GFP單克隆抗體、綠色熒光蛋白檢測、免疫印跡抗體、流式細胞術試劑、免疫熒光探針、重組蛋白檢測。
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基因名：GFP
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別名：CFP, eGFP, eYFP, GFP, GFP tag, YFP
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宿主：Mouse
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反應種屬：N/A
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免疫原：Recombinant GFP Protein
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標記方式：Non-conjugated
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克隆類型：Monoclonal Antibody
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抗體亞型：IgG2b
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純化方式：>95%,Protein A purified
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克隆號：6C11C11
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
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產品提供形式：liquid
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應用范圍：ELISA, WB, IF, FC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:50000-1:6400000 IF 1:50-1:200 FC 1:100-1:300 IP 1μl-2μl -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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