V5-Tag Monoclonal Antibody
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中文名稱：V5-Tag鼠單克隆抗體
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貨號：CSB-MA000160
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規格：￥1090
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圖片：
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IP antibody use: 5μg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with V5-tag fusion protein
Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose) -
IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
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1μg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
產品詳情
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產品描述：CUSABIO貨號：CSB-MA000160 V5-Tag單克隆抗體是針對重組蛋白研究中廣泛應用的V5表位標簽開發的科研專用試劑，該表位標簽源于副流感病毒血凝素神經氨酸酶蛋白，因其高特異性與低交叉反應特性成為蛋白標記及追蹤實驗的首選工具。本產品采用雜交瘤技術制備，經過ELISA、免疫印跡（WB）、免疫熒光（IF）和免疫沉淀（IP）等多平臺驗證，能精準識別帶有V5標簽的重組融合蛋白。其廣譜物種適用性支持跨物種樣本分析，適用于重組蛋白表達驗證、蛋白質相互作用網絡解析、亞細胞定位研究等實驗體系，尤其在多組學聯合分析中可配合His標簽或GST標簽抗體實現共表達蛋白的同步檢測。該抗體經嚴格質控確保批次穩定性，為基因功能研究、信號通路探索及蛋白結構解析提供高靈敏度的檢測解決方案，滿足分子生物學、細胞生物學等領域的科研需求。
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宿主：Mouse
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反應種屬：N/A
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免疫原：Synthetic Peptide
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標記方式：Non-conjugated
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抗體亞型：IgG
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克隆號：18G5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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