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中文名稱：TUBA1A鼠單克隆抗體
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貨號：CSB-MA754656A0m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg, 0.15625μg
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
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IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
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Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (5μg), Company B (5μg), Company C (5μg), Company D (5μg), CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
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Overlay histogram showing Hela cells stained with CSB-MA754656A0m (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay histogram showing Hela cells stained with Company A (5μg), Company B (5μg), Company C (5μg), Company D (5μg), CSB-MA754656A0m (5μg) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：TUBA1A單克隆抗體（CUSABIO貨號：CSB-MA754656A0m）是科研領域檢測微管蛋白α-1A鏈（TUBA1A）的高特異性工具，該靶標作為細胞骨架核心成分參與微管動態組裝、有絲分裂及神經元遷移調控，其功能異常與大腦皮質發育障礙及神經系統疾病密切相關。本產品采用雜交瘤技術制備，經嚴格驗證可識別人類、兔、大鼠及小鼠樣本中的TUBA1A蛋白，適用于多種實驗場景：通過Western Blot精準檢測細胞或組織裂解液中的TUBA1A表達水平，利用免疫組化（IHC）定位腦組織切片中的蛋白分布特征，結合免疫熒光（IF）觀察活細胞內微管網絡形態，或借助流式細胞術（FC）進行表面標記分析。在神經發育研究、細胞周期調控實驗及疾病模型構建中，該抗體可配合ELISA、免疫共沉淀（IP）等多元技術方案，為探索微管相關分子機制提供可靠支持，尤其適用于大腦畸形病理分析、神經元遷移障礙研究及神經退行性疾病機制等前沿科研領域。
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產品名稱：Mouse anti-Homo sapiens (Human) TUBA1A Monoclonal Antibody antibody
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Uniprot No.：
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基因名：
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別名：Alpha tubulin 3 antibody; Alpha-tubulin 3 antibody; B alpha 1 antibody; FLJ25113 antibody; LIS3 antibody; TBA1A_HUMAN antibody; TUBA1A antibody; TUBA3 antibody; Tubulin alpha 1a antibody; Tubulin alpha 1A chain antibody; Tubulin alpha 3 antibody; Tubulin alpha 3 chain antibody; Tubulin alpha brain specific antibody; Tubulin alpha-1A chain antibody; Tubulin alpha-3 chain antibody; Tubulin B alpha 1 antibody; Tubulin B-alpha-1 antibody
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宿主：Mouse
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反應種屬：Human, Rabbit, Rat, Mouse
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免疫原：A synthesized peptide derived from human Tubulin alpha-1A chain (297-309aa)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal Antibody
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抗體亞型：IgG2a
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純化方式：>95%, Protein A purified
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克隆號：7E5C12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:20000-1:320000 IHC 1:100-1:300 IF 1:50-1:200 FC 1:100-1:300 IP 1µg-5µg -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
產品評價
相關產品
靶點詳情
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功能：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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基因功能參考文獻：
- A de novo heterozygous c.320A>G [p.(His 107 Arg)] mutation in TUBA1A was identified in a patient with microcephaly, epileptic seizures, and severe developmental delay. PMID: 29109381
- Given that Spastin engages the MT in two places, we propose that severing occurs by forces exerted on the C-terminal tail of tubulin, which results in a conformational change in tubulin, which releases it from the polymer. PMID: 17389232
- Molecular docking studies revealed that 6f interacted and bound ef fi ciently with the colchicine-binding site of tubulin. In addition, 6f treatment induced G2/M cell cycle arrest dose-dependently and subsequently induced cell apoptosis PMID: 28440465
- induced pluripotent stem cells (iPSCs) from the umbilical cord and peripheral blood of two lissencephaly patients with different clinical severities carrying alpha tubulin (TUBA1A) missense mutations, were generated. PMID: 27431206
- Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing alpha-tubulin acetylation in breast cancer. PMID: 27228351
- Results show that Tuba1a plays an essential, noncompensated role in neuronal saltatory migration in vivo and highlight the importance of microtubule flexibility in nucleus-centrosome coupling and neuronal-branching regulation during neuronal migration. PMID: 28687665
- data suggest that the TUBA1A mutations disrupting lateral interactions have pronounced dominant-negative effects on microtubule dynamics that are associated with the severe end of the lissencephaly spectrum PMID: 26493046
- Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
- Data show that plasma membrane Ca(2+)-ATPase (PMCA) was associated with tubulin in normotensive and hypertensive erythrocytes. PMID: 26307527
- Studies indicate that alpha-tubulin acetylation and microtubule level is mainly governed by opposing actions of alpha-tubulin acetyltransferase 1 (ATAT1) and histone deacetylase 6 (HDAC6). PMID: 26227334
- Data from studies using peptide fragment of alpha-tubulin (residues 31-49) suggest that Ser38 is crucial for substrate recognition by alpha-tubulin acetylase 1 (ATAT1); Asp39, Ile42, the glycine stretch (residues 43-45), and Asp46 are also involved. PMID: 25602620
- Lysine 40 acetylation of alpha-tubulin does not result in significant changes in kinesin-1's landing rate or motility parameters. PMID: 24940781
- These results demonstrated that SelP interacts with tubulin, alpha 1a (TUBA1A). PMID: 24914767
- This study show all foetuses with lissencephaly and cerebellar hypoplasia carried distinct TUBA1A mutations. PMID: 25059107
- These findings call attention to PKC-mediated phosphorylation of alpha-tubulin as a novel mechanism for controlling the dynamics of microtubules that result in cell movement. PMID: 24574051
- case provides new insight into the wide spectrum of disease phenotypes associated with TUBA1A mutation PMID: 23528852
- The present study confirms that mutations in tubulin genes are responsible for complex brain malformation. PMID: 24392928
- Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
- Missense mutations in TUBA1A were found in 3 patients with polymicrogyria. PMID: 22948023
- We described the clinical course and pathological findings in a child with TUBA1A mutation PMID: 22633752
- TUBA1A and TUBB2B coding regions have been sequenced that are associated with cortical malformations. PMID: 23361065
- Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
- study describes a 14-month-old girl with TUBA1A mutation-associated lissencephaly, and summarize the clinical and neuroradiologic findings of 19 cases in the literature PMID: 22264709
- Alpha2B-adrenergic receptor interaction with tubulin controls its transport from the endoplasmic reticulum to the cell surface PMID: 21357695
- The expression of alpha-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung carcinoma. PMID: 20510079
- We report a mutation in TUBA1A as a cause of polymicrogyria. So far, all mutations in TUBA1A have occurred de novo, resulting in isolated cases. This article describes familial recurrence of TUBA1A mutations due to somatic mosaicism in a parent. PMID: 21403111
- Data show that IAV-infected cells contain elevated level of AcTub and alpha-tubulin. PMID: 21094644
- Mutations in TUBA1A result in defects in the tubulin folding and heterodimer assembly. PMID: 20603323
- LIS-associated mutations of TUBA1A operate via diverse mechanisms that include disruption of binding sites for microtubule-associated proteins. PMID: 20466733
- the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
- Mutations in alpha-tubulin in mice and humans that affect neuronal migration result in abnormal lamination of brain structures with associated behavioral deficits. PMID: 17218254
- Retrospective examination of MR images suggests that patients with TUBA1A mutations share not only cortical dysgenesis, but also cerebellar, hippocampal, corpus callosum, and brainstem abnormalities PMID: 17584854
- Increased expression of tubulin alpha is associated with pulmonary sclerosing hemangioma PMID: 17914564
- The diminished production of TUBA1A tubulin in R264C individuals is consistent with haploinsufficiency as a cause of the disease phenotype. PMID: 18199681
- the TUBA1A phenotype is distinct from LIS1, DCX, RELN and ARX lissencephalies. Compared with the phenotypes of children mutated for TUBA1A, these prenatally diagnosed fetal cases occur at the severe end of the TUBA1A lissencephaly spectrum. PMID: 18669490
- Missense mutations within the TUBA1A gene are associated with specific abnormalities in lissencephaly. PMID: 18728072
- mutation analysis in the TUBA1A gene in 46 patients with classical lissencephaly. PMID: 18954413
- This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
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相關疾病：Lissencephaly 3 (LIS3)
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亞細胞定位：Cytoplasm, cytoskeleton.
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蛋白家族：Tubulin family
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組織特異性：Expressed at a high level in fetal brain.
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