Myc-Tag Monoclonal Antibody
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中文名稱：Myc-Tag鼠單克隆抗體
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貨號：CSB-MA000157
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規(guī)格：￥1090
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圖片：
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IP antibody use: 3µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with C-Myc-tag fusion protein
Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 4: IP (transfected 293 + anti- C-Myc mAb + Protein G agarose)
Lane 5: IP (transfected 293 + Protein G)
Lane 6: Recombinant protein (E.coli) -
IF analysis of 293 cells transfected with a Myc-tag protein,1:2000 dilution (blue DAPI, red anti-Myc)
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1µg Myc fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA000157 Myc-Tag單克隆抗體是針對Myc表位標簽研發(fā)的高特異性科研工具，采用小鼠雜交瘤技術(shù)制備的IgG型單抗，可識別重組蛋白中插入的Myc短肽序列（EQKLISEEDL）。該產(chǎn)品經(jīng)免疫印跡、免疫熒光、免疫沉淀及ELISA多平臺驗證，1 mg/ml標準濃度確保實驗穩(wěn)定性，適用于哺乳動物、昆蟲、酵母及細菌等多種表達系統(tǒng)的重組蛋白檢測。抗體采用親和純化工藝制備，純度達95%以上，凍存于含50%甘油的PBS緩沖液中，建議-20℃分裝保存避免反復(fù)凍融。在蛋白質(zhì)相互作用研究、亞細胞定位分析及基因工程產(chǎn)物驗證等場景中表現(xiàn)優(yōu)異，特別適用于融合蛋白表達效率評估、蛋白復(fù)合物共沉淀研究及熒光標記定量檢測，其高親和力特性可有效降低非特異性背景信號，是分子生物學和細胞生物學研究的關(guān)鍵試劑。
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：Synthetic Peptide
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標記方式：Non-conjugated
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抗體亞型：IgG
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克隆號：25D6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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