E-Tag Monoclonal Antibody
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中文名稱：E-Tag鼠單克隆抗體
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貨號(hào)：CSB-MA000151M0m
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規(guī)格：￥400
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圖片：
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Western Blot
Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Western Blot
Positive WB detected in: E-tagged fusion protein
All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
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Immunoprecipitating E-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg) -
ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
Antigen at dilution of 1µg/ml
E-Tag antibody at (0-0.15µg)
Goat polyclonal to Mouse IgG at 1/50000 dilution -
Western Blot
Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號(hào)：CSB-MA000151M0m E-Tag單克隆抗體是針對(duì)重組蛋白研究中廣泛應(yīng)用的E-Tag標(biāo)簽開發(fā)的科研工具，該標(biāo)簽系統(tǒng)常用于目標(biāo)蛋白的追蹤、純化及互作研究。本產(chǎn)品采用高親和力小鼠源單抗制備，能特異性識(shí)別由GAPVPYPDPLEPR氨基酸序列組成的E-Tag表位，適用于各類物種來源的E-Tag標(biāo)記蛋白檢測。經(jīng)嚴(yán)格驗(yàn)證可在ELISA實(shí)驗(yàn)中實(shí)現(xiàn)精準(zhǔn)定量分析，在Western Blot中清晰呈現(xiàn)10-150 kDa分子量范圍內(nèi)的靶蛋白條帶，并支持免疫共沉淀（IP）技術(shù)用于蛋白相互作用研究。該抗體適用于重組蛋白表達(dá)驗(yàn)證、蛋白純化過程監(jiān)控以及蛋白質(zhì)相互作用組學(xué)等科研場景，特別在哺乳動(dòng)物表達(dá)系統(tǒng)、原核表達(dá)系統(tǒng)及體外翻譯系統(tǒng)的研究中展現(xiàn)優(yōu)異性能。為分子生物學(xué)、生物化學(xué)領(lǐng)域的E-Tag標(biāo)記蛋白研究提供穩(wěn)定可靠的檢測支持，助力重組蛋白功能解析及信號(hào)通路機(jī)制探索。
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別名：E-Tag
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宿主：Mouse
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反應(yīng)種屬：All
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免疫原：GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal Antibody
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號(hào)：11H12B3
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IP
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推薦稀釋比：
Application Recommended Dilution ELISA 1:4000-1:8000 WB 1:1000-1:320000 IP 1μg-5μg -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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