HEK293T/Human SARM1 Stable Cell Line

貨號：

CSB-SC750971HU
規格：

1 vial contains approximately 5x10⁶ cells in 1 ml
價格：

￥30000
活性：
Untransfected HEK293T cells (green line) and transfected Human SARM1 HEK293T Stable cells (red line) were stained with anti-Flag antibody (2μg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.

Western Blot
Positive WB detected in: Lane1：20 μg HEK293T cells, Lane2：40 μg Human SARM1 HEK293T Stable cells, Lane3：20 μg Human SARM1 HEK293T Stable cells
All lanes: Anti-Flag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 77.5 KDa
Observed band size: 77 KDa
其他：

產品詳情
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q6SZW1
生長類型：

Adherent
抗性：

Puromycin
培養基：

DMEM + 10% FBS + 2μg/mL Puromycin
別名：

SARM1
蛋白標簽：

C-terminal Flag-tagged
保存緩沖液：

35% FBS + 55% DMEM + 10% DMSO
應用范圍：

Binding assay by FACS and Western blot
儲存條件：

Frozen in liquid nitrogen or stored at -80°C
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

靶點詳情

功能：

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
基因功能參考文獻：
1. we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644
2. Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829
3. Data show that sterile alpha- and armadillo-motif-containing protein (SARM) modulates MyD88 protein-mediated Toll-like receptors (TLRs) activation through BB-loop dependent interleukin-1 receptor (TIR) TIR-TIR interactions. PMID: 26592460
4. These results indicate that association of PINK1 with SARM1 and TRAF6 is an important step for mitophagy. PMID: 23885119
5. The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling. PMID: 23923041
6. Rapid Wallerian degeneration requires the pro-degenerative molecules SARM1. PMID: 24840802
7. Data found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis. PMID: 24021647
8. SARM overexpression caused mitochondrial clustering which has also been observed in several cell death phenomenon. PMID: 23175186
9. The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856
10. SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472
11. Candidate gene in the onset of hereditary infectious/inflammatory diseases. PMID: 15893701
12. TIR adaptor SARM is a negative regulator of Toll-like receptor signaling. PMID: 16964262
13. confirmed the co-localization of retinoschisin with Na/K ATPase and SARM1 in photoreceptors and bipolar cells of retina tissue PMID: 17804407
14. SARM1 deficiencies may uncover unexpected similarities between the ways in which neurons and immune cells sense and respond to danger. PMID: 18089857
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亞細胞定位：

Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
組織特異性：

Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta.
數據庫鏈接：

HGNC: 17074

OMIM: 607732

KEGG: hsa:23098

STRING: 9606.ENSP00000406738

UniGene: Hs.743510

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