Recombinant Xenopus laevis Alpha-endosulfine (ensa)

中文名稱：

有爪蟾蜍ensa重組蛋白
貨號：

CSB-YP767253XBE
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

有爪蟾蜍ensa重組蛋白
貨號：

CSB-EP767253XBE
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

有爪蟾蜍ensa重組蛋白
貨號：

CSB-EP767253XBE-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

有爪蟾蜍ensa重組蛋白
貨號：

CSB-BP767253XBE
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

有爪蟾蜍ensa重組蛋白
貨號：

CSB-MP767253XBE
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ensa
Uniprot No.：

Q7ZXH9
別名：

ensaAlpha-endosulfine
種屬：

Xenopus laevis (African clawed frog)
蛋白長度：

full length protein
表達區域：

1-125
氨基酸序列

MSDKYIGDSH LEETGEEKQD SQEKEAVTPE KAEEQKLKAK YPNLGQKPGG SDFLMKRLQK GQKYFDSGDY NMAKAKIKNK QLPCAGPDKN LVTGDHIPTP QDLPQRKSSL VTSKLAGHVE DLHHV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with ppp2r2d (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase.
基因功能參考文獻：
1. description of how Gwl activation results in PP2A inhibition; Arpp19 and alpha-Endosulfine when phosphorylated by Gwl associate with and inhibit PP2A promoting mitotic entry; endogenous Arpp19 responsible for PP2A inhibition at mitotic entry in egg extracts PMID: 21164014
2. inhibition of PP2A-B55delta results from Ensa, that is phosphorylated in mitosis by the protein kinase Greatwall; this converts Ensa into specific inhibitor of PP2A-B55delta; this pathway represents a previously unknown element in mitosis control PMID: 21164013
亞細胞定位：

Cytoplasm.
蛋白家族：

Endosulfine family
數據庫鏈接：

KEGG: xla:379766

UniGene: Xl.84134