Recombinant Thermotoga maritima 5-keto-L-gluconate epimerase (iolO)

中文名稱：

海棲熱袍菌iolO重組蛋白
貨號：

CSB-EP893461TNJ
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

iolO
Uniprot No.：

Q9WYP7
別名：

Bifunctional nonphosphorylated sugar isomerase (D-erythrose/D-threose isomerase) (L-ribulose 3-epimerase) (R3E) (Nonphosphorylated sugar 3-epimerase) (Nonphosphorylated sugar aldose-ketose isomerase)
種屬：

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
蛋白長度：

Full Length
來源：

E.coli
分子量：

37.9 kDa
表達區域：

1-270aa
氨基酸序列

MKLSLVISTSDAAFDALAFKGDLRKGMELAKRVGYQAVEIAVRDPSIVDWNEVKILSEELNLPICAIGTGQAYLADGLSLTHPNDEIRKKAIERVVKHTEVAGMFGALVIIGLVRGRREGRSYEETEELFIESMKRLLELTEHAKFVIEPLNRYETDFINTIDDALRILRKINSNRVGILADTFHMNIEEVNIPESLKRAGEKLYHFHVADSNRWAPGCGHFDFRSVFNTLKEIGYNRYVSVECLPLPGGMEEAAEIAFKTLKELIIKLT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

13-23 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN. Is not active on the enantiomer 5-keto-D-gluconate. Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro. Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses. Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose. Exhibits no activity for C4-epimerization of D-tagatose to D-fructose.
基因功能參考文獻：
1. TM0416 is a putative nonphosphorylated sugar epimerase and catalyzes various C5 and C6 epimerization reactions. PMID: 28258150
蛋白家族：

Hyi family
數據庫鏈接：

KEGG: tma:TM0416

STRING: 243274.TM0416